Taxonomic characterization of Tanakia species (Acheilognathidae) using DNA barcoding analyses
| Autor: | Dacotah Anderson, Ho Young Suk, Hari Won, Hyung-Bae Jeon, Hangkyo Lim |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fish Proteins Mitochondrial DNA Range (biology) Tanakia Cyprinidae 010603 evolutionary biology 01 natural sciences DNA barcoding Electron Transport Complex IV 03 medical and health sciences Monophyly Genus Genetics Animals DNA Barcoding Taxonomic Molecular Biology Phylogeny biology Phylogenetic tree Cytochrome b Cytochromes b biology.organism_classification 030104 developmental biology Evolutionary biology |
| DOI: | 10.6084/m9.figshare.5584378 |
| Popis: | Tanakia is a bitterling genus with six species found in Far East Asia. Our aim was to construct the standard DNA barcode database available for the identification of six Tanakia species by comparing the range of intra- and inter-specific genetic distances, identifying the phylogenetic placement of each Tanakia species and providing the unique barcode characteristics that are specific to each species, using cytochrome oxidase I (COI) and cytochrome b (cyt b). Both loci failed to create a perfect barcoding gap between the ranges of inter- and intra-specific genetic distances, though interspecific COI distances were sufficiently greater than intraspecific values with only a few exceptions. In our phylogenetic analyses, T. koreensis and T. signifer did not form a monophyletic cluster of haplotypes in both loci. COI provided clear nucleotide characteristics that distinguish each species, whereas relatively fewer informative sites were found within the range of cyt b. Overall, COI could be regarded as appropriate species identification solution in Tanakia. Our analyses yielded some taxonomic issues that need the further investigation, and are expected to be helpful in the examination for the conservation status of Tanakia species that are on the verge of being endangered. |
| Databáze: | OpenAIRE |
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