Peripheral nerve regeneration by transplantation of bone marrow stromal cell—derived Schwann cells in adult rats
| Autor: | Hajime Sawada, Toshiro Mimura, Mari Dezawa, Isao Yamamoto, Hiroshi Kanno |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Pathology medicine.medical_specialty Time Factors Stromal cell Neural Conduction Nerve guidance conduit Schwann cell Walking Nerve conduction velocity medicine Animals Rats Wistar Bone Marrow Transplantation Matrigel business.industry Cell Differentiation Anatomy Sciatic Nerve Nerve Regeneration Rats Transplantation medicine.anatomical_structure Schwann Cells Sciatic nerve Bone marrow Stromal Cells business Follow-Up Studies |
| Zdroj: | Journal of Neurosurgery. 101:806-812 |
| ISSN: | 0022-3085 |
| DOI: | 10.3171/jns.2004.101.5.0806 |
| Popis: | Object. Bone marrow stromal cells (BMSCs) can be induced to form Schwann cells by sequentially treating the cells with β-mercaptoethanol and retinoic acid, followed by forskolin and neurotrophic factors including heregulin. In this study the authors made artificial grafts filled with BMSC-derived Schwann cells (BMSC-DSCs) and transplanted them into the transected sciatic nerve in adult rats to evaluate the potential of BMSCs as a novel alternative method of peripheral nerve regeneration. Methods. The BMSC-DSCs were suspended in Matrigel and transferred into hollow fibers (12 mm in length), which were transplanted into the transected sciatic nerve in adult Wistar rats. Six months after cell transplantation, electrophysiological evaluation and walking track analysis were performed. Results of these studies showed significant improvement in motor nerve conduction velocity and sciatic nerve functional index in the BMSC-DSC—transplanted group compared with the control group (Matrigel graft only). Immunohistochemical study data demonstrated that transplanted BMSCs labeled with retrovirus green fluorescent protein were positive for P0 and myelin-associated glycoprotein and had reconstructed nodes of Ranvier and remyelinated regenerated nerve axons. The number of regenerated axons in the axial section of the central portion of the graft was significantly greater in the transplanted group. Although BMSCs can differentiate into several types of cells, tumor formation did not occur 6 months after engraftment. Conclusions. Results in this study indicate that BMSC-DSCs have great potential to promote regeneration of peripheral nerves. The artificial graft made with BMSC-DSCs represents an alternative method for the difficult reconstruction of a long distance gap in a peripheral nerve. |
| Databáze: | OpenAIRE |
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