Changes in expression of serine biosynthesis and integrated stress response genes during myogenic differentiation of C2C12 cells
| Autor: | Congcong Li, Tim Parr, Paul T. Loughna, Madelaine C. Brearley, Zoe Daniel, John M. Brameld |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biophysics Biochemistry Serine lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Myosin medicine Integrated stress response lcsh:QD415-436 PHGDH lcsh:QH301-705.5 Myogenin Myogenesis dbcAMP Skeletal muscle Phosphoserine phosphatase Hypertrophy Cell biology 030104 developmental biology medicine.anatomical_structure chemistry lcsh:Biology (General) 030220 oncology & carcinogenesis Phosphoserine PCK2/PEPCK-M C2C12 Research Article |
| Zdroj: | Biochemistry and Biophysics Reports, Vol 20, Iss, Pp-(2019) Biochemistry and Biophysics Reports |
| ISSN: | 2405-5808 |
| Popis: | Skeletal muscle is a highly metabolic and dynamic tissue that is formed through the complex and well-organised process of myogenesis. Although there is a good understanding about the role of the Muscle Regulatory Factors during myogenesis, little is known about the potential interplay of other metabolic proteins. The aim of this study was to determine the endogenous mRNA expression profile for a novel group of genes, recently associated with β2-adrenergic agonist (BA) induced muscle hypertrophy in pigs [1], during myogenic differentiation in C2C12 cells and their response to dibutyryl cyclic-AMP (dbcAMP). These genes included mitochondrial phosphoenolpyruvate carboxykinase (PCK2/PEPCK-M), genes involved in serine biosynthesis (Phosphoglycerate dehydrogenase, PHGDH; Phosphoserine aminotransferase-1, PSAT1; Phosphoserine phosphatase, PSPH) and those involved in an integrated stress response (Asparagine synthetase, ASNS; Sestrin-2, SESN2; and Activating transcription factor-5, ATF5). A coordinated peak in endogenous PCK2, PHGDH, PSAT1, PSPH, ASNS, ATF5 and SESN2 mRNA expression was observed at day 2 of differentiation (P Highlights • ASNS, PCK2, PHGDH, PSAT1, PSPH show similar mRNA expression patterns in C2C12 cells. • Novel peak in C2C12 cell endogenous mRNA expression for all these genes at day 2. • Novel peak in mRNA expression at day 2 coincided with peak in Myogenin mRNA. • dbcAMP treatment from day 0 significantly increased both MyHC-IIB and PC mRNA. • ASNS, PCK2, PHGDH and PSAT1 mRNA was unaffected by dbcAMP treatment from day 0 or 4. |
| Databáze: | OpenAIRE |
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