Human stem cell decorated nanocellulose threads for biomedical applications
| Autor: | Henrikki Mertaniemi, Andres Sanz-Garcia, Antti Mäkitie, Marjo Yliperttula, Olli Ikkala, Jouni Partanen, Carmen Escobedo-Lucea, Carolina Gandía |
|---|---|
| Přispěvatelé: | Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Clinicum, Korva-, nenä- ja kurkkutautien klinikka, Nanobio Pharmaceutics, Drug Research Program, Biopharmaceutics Group |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Chronic wound
Materials science Nanofibers Biophysics Wound healing Bioengineering Inflammation 02 engineering and technology Mesenchymal Stem Cell Transplantation 010402 general chemistry 01 natural sciences Biomaterials Surgical applications Tensile Strength Materials Testing Cell Adhesion medicine Humans Cellulose Cell adhesion Cells Cultured Wet strength Tissue Scaffolds ta114 Mesenchymal stem cell 021001 nanoscience & nanotechnology Bandages 3. Good health 0104 chemical sciences 317 Pharmacy Mechanics of Materials Cell culture 216 Materials engineering Nanofiber Undifferentiated stem cells Ceramics and Composites Cellulose nanofibrils Mesenchymal stem cells medicine.symptom Stem cell 0210 nano-technology Biomedical engineering |
| Zdroj: | BIOMATERIALS. 82:208-220 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2015.12.020 |
| Popis: | Upon surgery, local inflammatory reactions and postoperative infections cause complications, morbidity, and mortality. Delivery of human adipose mesenchymal stem cells (hASC) into the wounds is an efficient and safe means to reduce inflammation and promote wound healing. However, administration of stem cells by injection often results in low cell retention, and the cells deposit in other organs, reducing the efficiency of the therapy. Thus, it is essential to improve cell delivery to the target area using carriers to which the cells have a high affinity. Moreover, the application of hASC in surgery has typically relied on animal-origin components, which may induce immune reactions or even transmit infections due to pathogens. To solve these issues, we first show that native cellulose nanofibers (nanofibrillated cellulose, NFC) extracted from plants allow preparation of glutaraldehyde cross-linked threads (NFC-X) with high mechanical strength even under the wet cell culture or surgery conditions, characteristically challenging for cellulosic materials. Secondly, using a xenogeneic free protocol for isolation and maintenance of hASC, we demonstrate that cells adhere, migrate and proliferate on the NFC-X, even without surface modifiers. Cross-linked threads were not found to induce toxicity on the cells and, importantly, hASC attached on NFC-X maintained their undifferentiated state and preserved their bioactivity. After intradermal suturing with the hASC decorated NFC-X threads in an ex vivo experiment, cells remained attached to the multifilament sutures without displaying morphological changes or reducing their metabolic activity. Finally, as NFC-X optionally allows facile surface tailoring if needed, we anticipate that stem-cell-decorated NFC-X opens a versatile generic platform as a surgical bionanomaterial for fighting postoperative inflammation and chronic wound healing problems. |
| Databáze: | OpenAIRE |
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