Sequencing of glycosaminoglycans with potential to interrogate sequence-specific interactions
| Autor: | Willeke F. Daamen, Els M.A. van de Westerlo, Emina Podhumljak, Elly M. M. Versteeg, Arie Oosterhof, Toin H. van Kuppevelt |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:Medicine Sequence (biology) Article Glycosaminoglycan 03 medical and health sciences chemistry.chemical_compound Carbohydrate Conformation Chondroitin sulfate lcsh:Science Glycosaminoglycans chemistry.chemical_classification Multidisciplinary 030102 biochemistry & molecular biology biology Chemistry Biomolecule lcsh:R Molecular biology carbohydrates (lipids) 030104 developmental biology Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] Proteoglycan Biochemistry Covalent bond biology.protein Nucleic acid lcsh:Q |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) Scientific Reports, 7 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-15009-0 |
| Popis: | Contains fulltext : 180507.pdf (Publisher’s version ) (Open Access) Technologies to sequence nucleic acids/proteins are widely available, but straightforward methodologies to sequence complex polysaccharides are lacking. We here put forward a strategy to sequence glycosaminoglycans, long linear polysaccharides involved in many biochemical processes. The method is based on the covalent immobilization and (immuno)chemical characterization of only those size-separated saccharides that harbor the original reducing end of the full-length chain. Using this methodology, the saccharide sequence of the chondroitin sulfate chain of the proteoglycan bikunin was determined. The method can be performed in any standard biochemical lab and opens studies to the interaction of complex saccharide sequences with other biomolecules. |
| Databáze: | OpenAIRE |
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