Development and validation of an UFLC-MS/MS method for enantioselectivity determination of d,l-thero-methylphenidate, d,l-thero-ethylphenidate and d,l-thero-ritalinic acid in rat plasma and its application to pharmacokinetic study
| Autor: | Yubo Wu, Junyun Zhang, Guobei Lin, Chenghao Zhang, Huafei Luo, Furong Zhang, Hao Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Clinical Biochemistry Tandem mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Ritalinic acid Analytical Chemistry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Limit of Detection Tandem Mass Spectrometry medicine Trifluoroacetic acid Animals Chromatography High Pressure Liquid Detection limit Chromatography 010401 analytical chemistry Extraction (chemistry) Reproducibility of Results Stereoisomerism Cell Biology General Medicine 0104 chemical sciences Rats Ethylphenidate chemistry Linear Models Methylphenidate Female Ammonium acetate medicine.drug |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1011 |
| ISSN: | 1873-376X |
| Popis: | A chiral UFLC-MS/MS method was established and validated for quantifying d-threo-methylphenidate (d-threo-MPH), l-threo-methylphenidate (l-threo-MPH), d-threo-ethylphenidate (d-threo-EPH), l-threo-ethylphenidate (l-threo-EPH) and d,l-threo-ritalinic acid (d,l-threo-RA) in rat plasma over the linearity range of 1-500ng/mL. Chiral separation was performed on an Astec Chirobiotic V2 column (5μm, 250×2.1mm) with isocratic elution using methanol containing 0.003% ammonium acetate (w/v) and 0.003% trifluoroacetic acid (v/v) at a flow of 0.3mL/min. All analytes and IS were extracted from rat plasma by a one-step liquid-liquid extraction (LLE) method. The intra- and inter-run accuracies were within 85-115%, and the intra- and inter-run precision were |
| Databáze: | OpenAIRE |
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