An objective and automated method for evaluating abdominal hyperalgesia in a rat model for endometriosis
| Autor: | Patrick G. Groothuis, Tamas Kozicz, Ard Peeters, Mieke van Aken, Marcel van Duin, Annemiek W. Nap, Tineke van Rijn, Didi D.M. Braat, Maria Panagiotou |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Endometriosis
Stress-related disorders Donders Center for Medical Neuroscience [Radboudumc 13] Abdominal cavity law.invention animal welfare 03 medical and health sciences 0302 clinical medicine Operant conditioning chamber Animal model law Abdomen medicine Animals pain Rats Wistar 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine General Veterinary business.industry Action intention and motor control animal model Chronic pain medicine.disease Rats Women's cancers Radboud Institute for Health Sciences [Radboudumc 17] behaviour Transplantation Disease Models Animal medicine.anatomical_structure Hyperalgesia Anesthesia Female Animal Science and Zoology medicine.symptom business |
| Zdroj: | Laboratory Animals. SAGE PUBLICATIONS INC Laboratory Animals Laboratory Animals, 54, 4, pp. 365-372 Laboratory Animals, 54, 365-372 |
| ISSN: | 0023-6772 |
| Popis: | Item does not contain fulltext Chronic pain and subfertility are the main symptoms of concern in women with endometriosis. In order to find new therapeutic options to suppress the pain, translational animal models are indispensable. We have developed a new automated, experimental setup, with full consideration for animal wellbeing, to determine whether operant behaviour can reveal abdominal hyperalgesia in rats with surgically-induced endometriosis, in order to assess whether abdominal hyperalgesia affect behavioural parameters. Endometriosis was induced by transplantation of uterine fragments in the abdominal cavity. Control groups consisted of sham-operated rats and non-operated rats. We have developed an operant chamber (Skinnerbox) which includes a barrier. The rat can climb the barrier in order to reach the food pellet, increasing in this way the pressure to the abdomen. We show that endometriosis rats collect significantly less sugar pellets when compared with the control rats after the introduction of the barrier. In the Skinnerbox experiment, we showed that in a positive operant setting, the introduction of a barrier results in a contrast of operant behaviour of endometriosis rats and control groups, perchance as a result of abdominal discomfort/hyperalgesia due to surgically-induced endometriosis. This is a promising start for the further development of a refined animal model to monitor abdominal discomfort/hyperalgesia in rats with surgically-induced endometriosis. 8 p. |
| Databáze: | OpenAIRE |
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