Collagen-induced rapid morphogenesis of human mammary epithelial cells: the role of the alpha 2 beta 1 integrin
| Autor: | Joyce Taylor-Papadimitriou, C Gilbert, Moira Shearer, F. Berdichevsky |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Integrins
medicine.drug_class Cellular differentiation Cell Integrin Morphogenesis Alpha (ethology) Biology Monoclonal antibody Cell Line Cell Movement medicine Cell Adhesion Humans Breast Cell adhesion Antibodies Monoclonal Cell Differentiation Epithelial Cells Cell Biology Cell biology medicine.anatomical_structure Cell culture Immunology biology.protein Collagen |
| Zdroj: | Journal of cell science. 102 |
| ISSN: | 0021-9533 |
| Popis: | The cell line MTSV1-7, originally derived by immortalizing mammary epithelial cells cultured from human milk was able to form three-dimensional structures in collagen gel. We have now found that these cells, cultured as a monolayer, are able to undergo rapid morphogenesis forming ridges and balls around collagen fibres, when soluble collagen type I is added to the medium. Monoclonal antibodies to the alpha 2 (P1E6) and beta 1- (mAB13) subunits of VLA-2, but not to the alpha 3-subunit (P1B5) of VLA-3, could block this collagen-induced rapid morphogenesis (CIRM). The effect of the antibodies on cell attachment, spreading, and migration on collagen gels was analyzed to identify alpha 2 beta 1 dependent steps which might be involved in CIRM. The results suggest that while other proteins, besides alpha 2 beta 1, are also involved in cell attachment and migration, cell spreading was specifically blocked by antibodies to the VLA-2, but not to the VLA-3 integrin. The results demonstrate that the alpha 2 beta 1 integrin plays a crucial role in the collagen-induced morphogenesis of human mammary epithelial cells and implicate the process of VLA-2-dependent cell spreading as an important step in this morphogenesis. |
| Databáze: | OpenAIRE |
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