A portable system for processing donated whole blood into high quality components without centrifugation

Autor: Fleur M. Aung, Gary D. Griffin, Eszter Vörös, Benjamin Lichtiger, Sean C. Gifford, Hui Xia, Briony C. Strachan, Kian Torabian, Sergey S. Shevkoplyas, Taylor A. Tomasino
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Blood transfusion
Platelet Aggregation
Physiology
medicine.medical_treatment
Microfluidics
lcsh:Medicine
Centrifugation
Blood Donors
030204 cardiovascular system & hematology
0302 clinical medicine
Animal Cells
Materials Physics
Blood plasma
Medicine and Health Sciences
Platelet
lcsh:Science
Whole blood
Multidisciplinary
Chemistry
Physics
Hematology
Body Fluids
Separation Processes
Blood
medicine.anatomical_structure
Physical Sciences
Engineering and Technology
Fluidics
Anatomy
Cellular Types
Sedimentation
Research Article
Platelets
Materials Science
Research and Analysis Methods
Blood Plasma
Specimen Handling
03 medical and health sciences
medicine
Humans
Platelet activation
Blood Coagulation
Blood Cells
lcsh:R
Biology and Life Sciences
Cell Biology
Platelet Activation
Red blood cell
030104 developmental biology
Apheresis
lcsh:Q
Biomedical engineering
Zdroj: PLoS ONE, Vol 13, Iss 1, p e0190827 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Background The use of centrifugation-based approaches for processing donated blood into components is routine in the industrialized world, as disparate storage conditions require the rapid separation of ‘whole blood’ into distinct red blood cell (RBC), platelet, and plasma products. However, the logistical complications and potential cellular damage associated with centrifugation/apheresis manufacturing of blood products are well documented. The objective of this study was to evaluate a proof-of-concept system for whole blood processing, which does not employ electromechanical parts, is easily portable, and can be operated immediately after donation with minimal human labor. Methods and findings In a split-unit study (n = 6), full (~500mL) units of freshly-donated whole blood were divided, with one half processed by conventional centrifugation techniques and the other with the new blood separation system. Each of these processes took 2–3 hours to complete and were performed in parallel. Blood products generated by the two approaches were compared using an extensive panel of cellular and plasma quality metrics. Comparison of nearly all RBC parameters showed no significant differences between the two approaches, although the portable system generated RBC units with a slight but statistically significant improvement in 2,3-diphosphoglyceric acid concentration (p < 0.05). More notably, several markers of platelet damage were significantly and meaningfully higher in products generated with conventional centrifugation: the increase in platelet activation (assessed via P-selectin expression in platelets before and after blood processing) was nearly 4-fold higher for platelet units produced via centrifugation, and the release of pro-inflammatory mediators (soluble CD40-ligand, thromboxane B2) was significantly higher for centrifuged platelets as well (p < 0.01). Conclusion This study demonstrated that a simple, passive system for separating donated blood into components may be a viable alternative to centrifugation—particularly for applications in remote or resource-limited settings, or for patients requiring highly functional platelet product.
Databáze: OpenAIRE
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