A botulinum neurotoxin-like function ofPotentilla chinensisextract that inhibits neuronal SNARE complex formation, membrane fusion, neuroexocytosis, and muscle contraction
| Autor: | Dae-Hyuk Kweon, Won-Seok Park, Keejung Yoon, Jin Kyu Choi, Sehyun Kim, Hyun-Ju Koh, Hong-Ju Shing, Chang-Hwa Jung, Paul Heo, Yoosoo Yang |
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| Rok vydání: | 2012 |
| Předmět: |
Botulinum Toxins
Cell Pharmaceutical Science Nerve Tissue Proteins Flowers Neurotransmission Biology Membrane Fusion PC12 Cells Synaptic Transmission Exocytosis Mice Norepinephrine chemistry.chemical_compound Drug Discovery medicine Animals Muscle Skeletal Receptor Neurotransmitter Neurons Pharmacology Mice Inbred ICR Plant Extracts Lipid bilayer fusion General Medicine Rats Cell biology medicine.anatomical_structure Lower Extremity Neuromuscular Agents Complementary and alternative medicine Biochemistry chemistry Potentilla Molecular Medicine Female medicine.symptom SNARE Proteins SNARE complex Muscle Contraction Muscle contraction |
| Zdroj: | Pharmaceutical Biology. 50:1157-1167 |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.3109/13880209.2012.661743 |
| Popis: | Botulinum neurotoxins (BoNTs) are popularly used to treat various diseases and for cosmetic purposes. They act by blocking neurotransmission through specific cleavage of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins. Recently, several polyphenols were shown to interfere with SNARE complex formation by wedging into the hydrophobic core interface, thereby leading to reduced neuroexocytosis.In order to find industrially-viable plant extract that functions like BoNT, 71 methanol extracts of flowers were screened and BoNT-like activity of selected extract was evaluated.After evaluating the inhibitory effect of 71 flower methanol extracts on SNARE complex formation, seven candidates were selected and they were subjected to SNARE-driven membrane fusion assay. Neurotransmitter release from neuronal PC12 cells and SNARE complex formation inside the cell was also evaluated. Finally, the effect of one selected extract on muscle contraction and digit abduction score was determined.The extract of Potentilla chinensis Ser. (Rosaceae)(Chinese cinquefoil) flower inhibited neurotransmitter release from neuronal PC12 cells by approximately 90% at a concentration of 10 μg/mL. The extract inhibited neuroexocytosis by interfering with SNARE complex formation inside cells. It reduced muscle contraction of phrenic nerve-hemidiaphragm by approximately 70% in 60 min, which is comparable to the action of the Ca²⁺-channel blocker verapamil and BoNT type A.While BoNT blocks neuroexocytosis by cleaving SNARE proteins, the Potentilla chinensis extract exhibited the same activity by inhibiting SNARE complex formation. The extract paralyzed muscle as efficiently as BoNT, suggesting the potential versatility in cosmetics and therapeutics. |
| Databáze: | OpenAIRE |
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