Human cytomegalovirus particles directly suppress CD4 T-lymphocyte activation and proliferation

Autor: Inti Peredo, Giuseppe Stragliotto, Olesja Fornara, Zahidul Khan, Lynn M. Butler, Jenny Odeberg, Cecilia Söderberg-Nauclér
Rok vydání: 2013
Předmět:
Antigens
Differentiation
T-Lymphocyte
CD4-Positive T-Lymphocytes
Human cytomegalovirus
Herpesvirus 2
Human
viruses
Immunology
Cytomegalovirus
chemical and pharmacologic phenomena
Herpesvirus 1
Human
Antibodies
Viral
Lymphocyte Activation
Measles virus
Interferon-gamma
Immune system
Antigens
CD
Cell Line
Tumor
Concanavalin A
medicine
Humans
Immunology and Allergy
Lectins
C-Type
Phytohemagglutinins
Cell Proliferation
chemistry.chemical_classification
biology
Tumor Necrosis Factor-alpha
Chemistry
Macrophages
CD69
virus diseases
Hematology
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
medicine.disease
Apoptosis
Cytomegalovirus Infections
Leukocytes
Mononuclear
biology.protein
Leukocyte Common Antigens
Tetradecanoylphorbol Acetate
Interleukin-4
Antibody
K562 Cells
Glycoprotein
Zdroj: Immunobiology. 218:1034-1040
ISSN: 0171-2985
DOI: 10.1016/j.imbio.2013.01.002
Popis: CD4 T cells are important regulators of the immune system and are vital for mounting a strong immune response against viral infections. Human cytomegalovirus (HCMV) is known to be a strong modulator of the innate as well as adaptive immune responses. In this study, we found that HCMV directly inhibited proliferation of CD4 T cells and rendered them unresponsive to immunological stimuli. This effect was not observed when CD4 T cells were treated with herpes simplex virus-1/2 or measles virus. When stimulated with phytohemagglutinin, concanavalin A, or phorbol myristate acetate, HCMV-treated T cells were unable to proliferate, revealing an ability of HCMV to inhibit CD4 T cell response. Furthermore, HCMV also prevented proliferation of leukemic T-cell lines. HCMV-treated CD4 T cells expressed the activation markers CD45RO and CD69, were not apoptotic and produced decreased levels of the cytokines IL-4, IFN-γ and TNF-α, compared to untreated controls. The inhibitory effect of HCMV on CD4 T cell proliferation was not mediated by HCMV gH, gB or other immunogenic glycoproteins, since intravenous immunoglobulins or gB- or gH-specific neutralizing antibodies did not prevent the suppression of T-cell proliferation. Our observations show that HCMV inhibits CD4 T cell function with potential clinical consequences for both humoral and cell-mediated immune responses.
Databáze: OpenAIRE
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