Impact of Cryopreservation on Motile Subpopulations and Tyrosine-Phosphorylated Regions of Ram Spermatozoa during Capacitating Conditions
| Autor: | Manuel Ramón, Ana J. Soler, María Rocío Fernández-Santos, Alicia Martín Maestro, Vidal Montoro, Patricia Peris-Frau, Daniela Alejandra Medina-Chávez, Irene Sánchez-Ajofrín, Olga García-Álvarez, C. Maside, José Julián Garde |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Universidad de Castilla La Mancha |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
QH301-705.5 Semen Biology Article General Biochemistry Genetics and Molecular Biology Cryopreservation sperm capacitation Tyrosine phosphorylation Andrology chemistry.chemical_compound Capacitation Sperm cryopreservation Tyrosine Biology (General) subpopulations Sperm motility reproductive and urinary physiology General Immunology and Microbiology urogenital system tyrosine phosphorylation computer-assisted sperm analysis Sperm capacitation cytometry Sperm Computer-assisted sperm analysis sperm cryopreservation chemistry Subpopulations Phosphorylation General Agricultural and Biological Sciences Cytometry |
| Zdroj: | Biology, Vol 10, Iss 1213, p 1213 (2021) Biology Volume 10 Issue 11 Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 2079-7737 |
| Popis: | This article belongs to the Special Issue Factors Affecting In Vitro Assessment of Sperm Quality. The heterogeneous nature of ejaculates highlights the relevance of studying the behavior of different sperm subpopulations. Changes in sperm motility and the increase in tyrosine phosphorylation are key events that usually occur during capacitation and can be modified by the cryopreservation process. However, the relationship between both events remains poorly defined throughout capacitation in the different sperm subpopulations. Fresh and frozen-thawed spermatozoa were incubated in capacitating (CAP) and non-capacitating (NC) media up to 240 min. Sperm kinematics, tyrosine phosphorylation and mitochondrial activity were measured by the CASA system and imaging flow cytometry. Four motile sperm subpopulations (SP) were identified in fresh and frozen-thawed ram semen after the cluster analysis. Incubation under CAP conditions over time led to greater changes in the percentage of spermatozoa included in each subpopulation compared to NC conditions, being different between fresh and frozen-thawed spermatozoa. The SP1, characterized by slow spermatozoa, progressively increased after 15 min in frozen-thawed samples incubated in both media but not in fresh ones. The SP4, characterized by fast and non-linear spermatozoa, showed a marked increase during CAP, but not under NC conditions, occurring more rapidly in frozen-thawed spermatozoa. This subpopulation (SP4) was also the only one positively and strongly correlated with mitochondrial activity and all phosphorylated sperm regions during capacitation, either in fresh or frozen-thawed samples. Our results indicated that in vitro capacitation induced significant changes in the distribution of motile sperm subpopulations, being affected by cryopreservation. Notwithstanding, the subpopulation which probably represents hyperactivated-like spermatozoa (SP4) also increased in frozen-thawed samples, occurring faster and simultaneously to the increment of mitochondrial activity and tyrosine phosphorylation of different sperm regions. This research was supported by the Spanish Ministry of Economy and Competitiveness (AGL2013-48421-R and AGL2017-89017-R). A.M.M. was supported by a Ministry of Economy and Competitiveness scholarship and P.P.-F. was supported by a University of Castilla-La Mancha scholarship [2015/4062]. |
| Databáze: | OpenAIRE |
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