Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma
| Autor: | Erdi Özdemir, Emine Kilic, Ramin Hashemihesar, Ali Celalettin Sinan Yuruker, Duygu Uçkan Çetinkaya, Abdülsamet Emet, Egemen Turhan |
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| Rok vydání: | 2020 |
| Předmět: |
Cartilage
Articular endocrine system Pathology medicine.medical_specialty Scaffold Placenta 0206 medical engineering Biomedical Engineering Medicine (miscellaneous) 02 engineering and technology 03 medical and health sciences Pregnancy Articular cartilage repair Animals Humans Regeneration Medicine 030304 developmental biology 0303 health sciences Decellularization Platelet-Rich Plasma business.industry Hyaline cartilage Cartilage Regeneration (biology) Mesenchymal stem cell Mesenchymal Stem Cells 020601 biomedical engineering Rats medicine.anatomical_structure Platelet-rich plasma Original Article Female business |
| Zdroj: | Tissue Eng Regen Med |
| ISSN: | 2212-5469 1738-2696 |
| Popis: | BACKGROUND: Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. METHODS: An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 × 10(6) MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n = 14). The DHP scaffold, 1 × 10(6) MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. RESULTS: The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p 0.05). CONCLUSION: In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration. |
| Databáze: | OpenAIRE |
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