Monoclonal antibodies specific for sickle cell hemoglobin
| Autor: | Ronald H. Jensen, Elbert Branscomb, R J Grabske, William L. Bigbee, Martin Vanderlaan, Larry H. Stanker |
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| Rok vydání: | 1985 |
| Předmět: |
Immunogen
medicine.drug_class Clinical Biochemistry Hemoglobin Sickle Enzyme-Linked Immunosorbent Assay Antigen-Antibody Complex Biology Monoclonal antibody Mice medicine Animals Humans Globin Amino Acid Sequence Peptide sequence Genetics (clinical) chemistry.chemical_classification Mice Inbred BALB C Biochemistry (medical) Antibodies Monoclonal Hematology Flow Cytometry Molecular biology Amino acid Biochemistry chemistry Monoclonal biology.protein Electrophoresis Polyacrylamide Gel Hemoglobin Antibody |
| Zdroj: | Hemoglobin. 9(4) |
| ISSN: | 0363-0269 |
| Popis: | Two mouse hybridoma cell lines were isolated which produce monoclonal antibodies that bind hemoglobin S. The mice were immunized with peptide-protein conjugates to stimulate a response to the amino terminal peptide of the beta chain of hemoglobin S, where the single amino acid difference between A and S occurs. Immunocharacterization of the antibodies shows that they bind specifically to the immunogen peptide and to hemoglobin S. The specificity for S is high enough that one AS cell in a mixture with a million AA cells is labeled by antibody, and such cells can be analyzed by flow cytometry. Immunoblotting of electrophoretic gels allows definitive identification of hemoglobin S as compared with other hemoglobins with similar electrophoretic mobility. |
| Databáze: | OpenAIRE |
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