Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia

Autor: Osman Hashim, Leo L.M. Poon, Malik Peiris, Ali Al-Doweriej, Ali Al-Hufofi, Samy Kasem, Ali Alkarar, Nasereldeen Babekr, Daniel K.W. Chu, Raed Hussien, Ali AL-Sahaf, Ranawaka A.P.M. Perera, Albagir Gaafer, Faisal Bayoumi, Ahmed A. Zaki, Ibraheem Qasim, Ali Abu-Obeida, Nadr El-Harby, Abdelhamid Elfadil, Ahmed Al-Romaihi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Veterinary medicine
Disease reservoir
viruses
medicine.disease_cause
GIZ
Gizan
Antibodies
Viral
ABT
Bahaa
MJH
Majmaa
MED
Al-Madina
0302 clinical medicine
RUH
Riyadh
Zoonoses
RAH
Northern Boundaries
030212 general & internal medicine
Phylogeny
Coronavirus
Real time-PCR
Goats
lcsh:Public aspects of medicine
TUU
Tabuk
virus diseases
MERS-CoV
Middle East respiratory syndrome coronavirus
General Medicine
Middle Aged
Infectious Diseases
SHG
Shagraa
TIF
Taif
EAM
Najran
Middle East Respiratory Syndrome Coronavirus
RNA
Viral
ELISA
AHB
Asir
Antibody
AKH
Alkharj
HOF
Ihsaa
Coronavirus Infections
DMM
Shargia
endocrine system
Camelus
Middle East respiratory syndrome coronavirus
WAE
Wadi El-Dwasir
Saudi Arabia
Enzyme-Linked Immunosorbent Assay
Biology
Real-Time Polymerase Chain Reaction
Virus
Article
DWD
El-Dowadmi
ZUL
Zulfi
lcsh:Infectious and parasitic diseases
03 medical and health sciences
Antigen
MERS
Dromedary camel
medicine
Animals
Humans
lcsh:RC109-216
Disease Reservoirs
Sheep
Whole Genome Sequencing
AJF
Jouf
ELQ
El-Qassim
MERS
Middle East respiratory syndrome
MAK
Makkah
Public Health
Environmental and Occupational Health
lcsh:RA1-1270
medicine.disease
Virology
030104 developmental biology
Cross-Sectional Studies
Immunoglobulin G
Herd
biology.protein
Middle East respiratory syndrome
Cattle
HAS
Hail
Zdroj: Journal of Infection and Public Health, Vol 11, Iss 3, Pp 331-338 (2018)
Journal of Infection and Public Health
ISSN: 1876-0341
Popis: Background: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. Methods: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014–2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed. Results: The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula. Conclusions: These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS. Keywords: MERS, Dromedary camel, Real time-PCR, ELISA, Saudi Arabia
Databáze: OpenAIRE
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