Association of TNF-α polymorphisms (−857, −863 and −1031), TNF-α serum level and lipid profile with acne vulgaris
| Autor: | Kanwal Nisar, Sara Mumtaz, Sana Shamim, Miroslav Blumenberg, Sidra Younis, Farah Deeba, Sabba Mehmood, Qamar Javed |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Linkage disequilibrium IL-1 Interleukin-1 Apolipoprotein B Acne severity QH301-705.5 Population AV Acne vulgaris TNFAlpha LGL Low glycemic load Association chemistry.chemical_compound High-density lipoprotein Polymorphism (computer science) Internal medicine Medicine Lipid Profile TNF-α Tumor necrosis factor alpha Acne vulgaris Biology (General) education TC Total cholesterol OPD Out-patient department Acne education.field_of_study biology medicine.diagnostic_test business.industry Cholesterol HDL-C High density lipoprotein cholesterol medicine.disease Endocrinology chemistry biology.protein Original Article C. acnes Cutibacterium acnes General Agricultural and Biological Sciences business Lipid profile Polymorphisms Apo-a Apolipoprotein a Acne types |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 28, Iss 11, Pp 6615-6620 (2021) Saudi Journal of Biological Sciences |
| Popis: | Background Acne is an inflammatory condition principally affected by genetic and dietary factors. Investigation into functional polymorphisms of TNF-α gene and their association with acne vulgaris will be helpful in exploring genetic influence on skin immune mediated inflammatory events. In the present study, we analyzed association of TNF-α gene polymorphisms, its expression levels and lipid profiles in a large cohort of acne patients and controls. Methods We used PCR-RFLP to study association of TNF-α polymorphisms at -857C/T, -863C/A and -1031 T/C sites with acne vulgaris. Lipid profiles were measured using enzymatic end-point method. The serum levels of TNF-α and apolipoprotein a were measured using ELISA. NIH, LDlink was used to investigate patterns of linkage disequilibrium across south Asian reference genome (Punjabi from Lahore Pakistan). Results We found that TNF-α -863 polymorphism is strongly associated with acne in overall population as well as in gender and severity based groups of acne patients. Polymorphisms at -863 and -1031 position were in linkage disequilibrium. Importantly, TNF-α serum level was significantly increased in acne patients with severe disease symptoms. Furthermore, levels of total cholesterol (TC) and triglycerides (TG) were significantly increased, whereas high density lipoprotein cholesterol (HDL-C) level was significantly decreased in acne patients. The levels of apolipoprotein a varied widely in studied populations and no significant difference was found in the analyzed groups. Conclusion In conclusion, we found that TNF-α expression increases in acne patients affected by TNF-α polymorphisms, and that the lipid profile is specifically disrupted in acne patients. |
| Databáze: | OpenAIRE |
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