Further evidence for a gamma/delta T cell receptor-mediated TCT.1/CD48 recognition
| Autor: | MAMI CHOUAIB, F, DEL PORTO, Paola, Delorme, D, Hercend, T. |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cytotoxicity
Immunologic Base Sequence Gene Rearrangement gamma-Chain T-Cell Antigen Receptor Gene Rearrangement delta-Chain T-Cell Antigen Receptor Molecular Sequence Data Immunology Receptors Antigen T-Cell gamma-delta CD48 Antigen In Vitro Techniques Clone Cells Structure-Activity Relationship Antigens CD Humans Immunology and Allergy Amino Acid Sequence T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of Immunology. 147:2864-2867 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.147.9.2864 |
| Popis: | We have demonstrated recently that a molecule, termed TCT.1 (Blast-1/CD48), is recognized on the surface of target cells by a series of alloreactive gamma/delta T cell clones generated from PBL of one healthy individual (designated E). Southern blot analyses suggested that these clones express a TCR associating a V3-JP2-C2 gamma-chain and V1-D-J1-C delta-chain. In the present study, we have developed from PBL of a second normal donor (designated G) a novel series of gamma/delta cloned T cell lines with similar functional activity (i.e., specific recognition of TCT.1 protein). The TCR gamma- and delta-chain nucleotide sequences of both the E and G clones were determined. Results show that 1) sequences from all the clones are identical in each individual donor, 2) the delta-chains expressed by the E and the G clones are encoded by distinct gene rearrangements including V1-D-J delta 1 and V1-D-J delta 2, respectively, 3) the gamma-chains expressed by the E and the G clones are encoded by the same genomic variable elements, namely V gamma 3 and JP2, whereas the junctional regions are distinct. Because the latter rearrangement is very infrequent in human peripheral blood, these data support the view that TCT.1/CD48 recognition is likely to be TCR dependent. |
| Databáze: | OpenAIRE |
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