Antimicrobial and antibiofilm activity of the benzoquinone oncocalyxone A
| Autor: | Tatiane Caroline Daboit, Otília Deusdênia L. Pessoa, Raí Emanuel da Silva, Fábio de Oliveira Silva Ribeiro, Alyne Rodrigues de Araújo, Maria José dos Santos Soares, José Roberto S. A. Leite, Andressa Maria Aguiar de Carvalho, Taynara Simão Matos, Jose Delano Barreto Marinho-Filho |
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| Rok vydání: | 2020 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine 030106 microbiology Anthraquinones Microbial Sensitivity Tests Gram-Positive Bacteria Microbiology 03 medical and health sciences Minimum inhibitory concentration Antibiotic resistance Staphylococcus epidermidis Gram-Negative Bacteria Benzoquinones Humans Minimum bactericidal concentration biology Chemistry Biofilm Antimicrobial biology.organism_classification Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Biofilms Bacterial cellular morphologies Bacteria |
| Zdroj: | Microbial Pathogenesis. 149:104513 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2020.104513 |
| Popis: | Resistance to antimicrobials is a challenging issue that complicates the treatment of infections caused by bacteria and fungi, thus requiring new therapeutic options. Oncocalyxone A, a benzoquinone obtained from Auxemma oncocalyx (Allem) Taub has several biological effects; however, there is no data on its antimicrobial action. In this study, its antimicrobial and antibiofilm activities were evaluated against bacteria and fungi of clinical interest. Strains of Gram-positive and Gram-negative bacteria, and filamentous fungi and yeasts were selected to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of oncocalyxone A. The antibacterial effect of oncocalyxone A was studied using survival curves, atomic force microscopy (AFM), and the involvement of oxidative stress. We examined the inhibitory action of the molecule on biofilm formation and its hemolytic activity against human erythrocytes. Our results showed that among the strains tested, Staphylococcus epidermidis was highly sensitive to the action of oncocalyxone A, with an MIC of 9.43 μg/mL. In most bacterial strains analyzed, a bacteriostatic effect was observed, though the molecule showed no antifungal activity. Antibiofilm activity was observed against the methicillin-resistant S. aureus bacteria. Additionally, results from atomic force microscopy imaging showed that oncocalyxone A significantly altered bacterial morphology. Further, oncocalyxone A showed no hemolytic activity at concentrations ≥151 μg/mL. Together, our results demonstrate the antibacterial and antibiofilm potential of oncocalyxone A, indicating its therapeutic potential against bacterial resistance. |
| Databáze: | OpenAIRE |
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