Genetic dissection of flowering time in flax (Linum usitatissimum L.) through single- and multi-locus genome-wide association studies
| Autor: | Sylvie Cloutier, Braulio J. Soto-Cerda, Gabriela Aravena |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Linum Candidate gene Time Factors Quantitative Trait Loci Single-nucleotide polymorphism Genome-wide association study Locus (genetics) Biology Quantitative trait locus Genes Plant 01 natural sciences Linkage Disequilibrium 03 medical and health sciences Gene Expression Regulation Plant Flax Genetics Allele Flowering Tops Molecular Biology Gene Expression Regulation Developmental food and beverages Sequence Analysis DNA General Medicine biology.organism_classification Genetic architecture 030104 developmental biology Genetic Loci Seeds Genome-Wide Association Study 010606 plant biology & botany |
| Zdroj: | Molecular Genetics and Genomics. 296:877-891 |
| ISSN: | 1617-4623 1617-4615 |
| Popis: | In a rapidly changing climate, flowering time (FL) adaptation is important to maximize seed yield in flax (Linum usitatissimum L.). However, our understanding of the genetic mechanism underlying FL in this multipurpose crop remains limited. With the aim of dissecting the genetic architecture of FL in flax, a genome-wide association study (GWAS) was performed on 200 accessions of the flax core collection evaluated in four environments. Two single-locus and six multi-locus models were applied using 70,935 curated single nucleotide polymorphism (SNP) markers. A total of 40 quantitative trait nucleotides (QTNs) associated with 27 quantitative trait loci (QTL) were identified in at least two environments. The number of QTL with positive-effect alleles in accessions was significantly correlated with FL (r = 0.77 to 0.82), indicating principally additive gene actions. Nine QTL were significant in at least three of the four environments accounting for 3.06-14.71% of FL variation. These stable QTL spanned regions that harbored 27 Arabidopsis thaliana and Oryza sativa FL-related orthologous genes including FLOWERING LOCUS T (Lus10013532), FLOWERING LOCUS D (Lus10028817), transcriptional regulator SUPERMAN (Lus10021215), and gibberellin 2-beta-dioxygenase 2 (Lus10037816). In silico gene expression analysis of the 27 FL candidate gene orthologous suggested that they might play roles in the transition from vegetative to reproductive phase, flower development and fertilization. Our results provide new insights into the QTL architecture of flowering time in flax, identify potential candidate genes for further studies, and demonstrate the effectiveness of combining different GWAS models for the genetic dissection of complex traits. |
| Databáze: | OpenAIRE |
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