Assessment of an Antibody-in-Lymphocyte Supernatant Assay for the Etiological Diagnosis of Pneumococcal Pneumonia in Children

Autor: Michael J. Carter, Pallavi Gurung, Claire Jones, Shristy Rajkarnikar, Rama Kandasamy, Meeru Gurung, Stephen Thorson, Madhav C. Gautam, Krishna G. Prajapati, Bibek Khadka, Anju Maharjan, Julian C. Knight, David R. Murdoch, Thomas C. Darton, Merryn Voysey, Brian Wahl, Katherine L. O'Brien, Sarah Kelly, Imran Ansari, Ganesh Shah, Nina Ekström, Merit Melin, Andrew J. Pollard, Dominic F. Kelly, Shrijana Shrestha
Rok vydání: 2019
Předmět:
0301 basic medicine
Serotype
Male
lcsh:QR1-502
lcsh:Microbiology
pneumococcus (Streptococcus pneumoniae)
Cellular and Infection Microbiology
diagnostic test (MeSH)
antibodies
Lymphocytes
Prospective Studies
Child
Pathogen
Original Research
biology
Antibodies
Bacterial
3. Good health
Infectious Diseases
C-Reactive Protein
Streptococcus pneumoniae
Child
Preschool
Pneumococcal pneumonia
Female
Antibody
Microbiology (medical)
Adolescent
030106 microbiology
Immunology
Immunologic Tests
Microbiology
Peripheral blood mononuclear cell
Sensitivity and Specificity
Virus
03 medical and health sciences
Antigen
Bacterial Proteins
Nepal
medicine
pneumonia
Humans
Antigens
Bacterial
business.industry
Diagnostic Tests
Routine
Infant
Pneumonia
Pneumococcal
medicine.disease
respiratory tract diseases
Pneumonia
030104 developmental biology
Immunoglobulin G
biology.protein
Leukocytes
Mononuclear
business
Zdroj: Frontiers in Cellular and Infection Microbiology
King's College London
Frontiers in Cellular and Infection Microbiology, Vol 9 (2020)
ISSN: 2235-2988
Popis: New diagnostic tests for the etiology of childhood pneumonia are needed. We evaluated the antibody-in-lymphocyte supernatant (ALS) assay to detect immunoglobulin (Ig) G secretion from ex vivo peripheral blood mononuclear cell (PBMC) culture, as a potential diagnostic test for pneumococcal pneumonia. We enrolled 348 children with pneumonia admitted to Patan Hospital, Kathmandu, Nepal between December 2015 and September 2016. PBMCs sampled from participants were incubated for 48 h before harvesting of cell culture supernatant (ALS). We used a fluorescence-based multiplexed immunoassay to measure the concentration of IgG in ALS against five conserved pneumococcal protein antigens. Of children with pneumonia, 68 had a confirmed etiological diagnosis: 12 children had pneumococcal pneumonia (defined as blood or pleural fluid culture-confirmed; or plasma CRP concentration ≥60 mg/l and nasopharyngeal carriage of serotype 1 pneumococci), and 56 children had non-pneumococcal pneumonia. Children with non-pneumococcal pneumonia had either a bacterial pathogen isolated from blood (six children); or C-reactive protein
Databáze: OpenAIRE
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