Selective isolation of poliovirus in recombinant murine cell line expressing the human poliovirus receptor gene
| Autor: | M Stenvik, T Hovi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Microbiology (medical)
viruses Gene Expression Biology Virus Replication medicine.disease_cause complex mixtures Virus Cell Line Microbiology law.invention Mice Cytopathogenic Effect Viral law Virology medicine Animals Humans Receptor Gene Cytopathic effect Poliovirus Membrane Proteins Evaluation Studies as Topic Cell culture Poliovirus Vaccine Oral Recombinant DNA Receptors Virus Enterovirus Research Article Poliomyelitis |
| Zdroj: | Journal of Clinical Microbiology. 32:1366-1368 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.32.5.1366-1368.1994 |
| Popis: | Sixty-eight laboratory strains representing 49 enterovirus, 10 adenovirus, and 3 reovirus serotypes were inoculated in a recombinant murine cell line expressing the human poliovirus receptor gene (L alpha cells). Only polioviruses caused cytopathic effect over a 10-day period. Likewise, only polioviruses were isolated, by use of L alpha cells, from 168 fecal specimens from children from developing countries. These results suggest that the recombinant L alpha cells can be used for selective isolation of poliovirus from clinical specimens. |
| Databáze: | OpenAIRE |
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