Potential Anticancer Mechanisms of a Novel EGFR/DNA-Targeting Combi-Molecule (JDF12) against DU145 Prostate Cancer Cells: An iTRAQ-Based Proteomic Analysis
| Autor: | Haofeng Zheng, Guancan Liang, Youqiang Fang, Wei Liu, Yanxiong Chen, Sijie Lin |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Article Subject lcsh:Medicine Antineoplastic Agents Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Prostate cancer 0302 clinical medicine DU145 Annexin Cell Line Tumor medicine Humans MTT assay Molecular Targeted Therapy Protein Interaction Maps Propidium iodide KEGG General Immunology and Microbiology lcsh:R Prostatic Neoplasms General Medicine medicine.disease Molecular biology Neoplasm Proteins ErbB Receptors Gene Expression Regulation Neoplastic 030104 developmental biology Real-time polymerase chain reaction chemistry 030220 oncology & carcinogenesis Cancer research Signal transduction Research Article Signal Transduction |
| Zdroj: | BioMed Research International, Vol 2017 (2017) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | The development of multitargeting drugs is an emerging trend in cancer research. To promote further development and clinical application of multitargeting drugs, this research was performed. MTT assay and flow cytometry of Annexin V/propidium iodide staining were used to confirm the proapoptotic efficacy of a novel combi-targeting molecule, JDF12, against DU145 prostate cancer (PCa) cells. Differentially expressed proteins between control and JDF12-treated cultures were revealed by isobaric tags for relative and absolute quantitation (iTRAQ), and part of them was confirmed by quantitative PCR. Differentially expressed proteins were further analyzed for function, pathway association, and protein−protein interactions using GO, KEGG, and STRING databases. A total of 119 differentially expressed proteins, 70 upregulated and 49 downregulated, were implicated in the anticancer effects of JDF12. Many of these proteins are involved in biosynthesis, response to stress, energy metabolism, and signal transduction. This study provides important information for understanding the anti-PCa mechanisms of JDF12, and well-designed combi-targeting drugs may possess stronger anticancer efficacy than single-targeting drugs and are thus promising candidates for clinical application. |
| Databáze: | OpenAIRE |
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