lncRNA Sensing of a Viral Suppressor of RNAi Activates Non-canonical Innate Immune Signaling in Drosophila
| Autor: | Xinlei Gao, Dongyang Guo, Liqin Zhang, Olugbenga Emmanuel Ajayi, Wenjie Li, Qingfa Wu, Shou-Wei Ding, Shuishui Qi, Wen Xu |
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| Rok vydání: | 2019 |
| Předmět: |
Virulence Factors
Microbiology Virus 03 medical and health sciences Viral Proteins 0302 clinical medicine Ubiquitin RNA interference Immunity Virology Animals Drosophila Proteins Gene 030304 developmental biology 0303 health sciences Innate immune system biology fungi RNA biology.organism_classification Immunity Innate Cell biology Drosophila melanogaster Gene Knockdown Techniques biology.protein Dicistroviridae Parasitology RNA Interference RNA Long Noncoding CRISPR-Cas Systems Carrier Proteins Drosophila C virus 030217 neurology & neurosurgery Antimicrobial Cationic Peptides Signal Transduction |
| Zdroj: | Cell hostmicrobe. 27(1) |
| ISSN: | 1934-6069 |
| Popis: | Antiviral immunity in insects is mediated by the RNA interference (RNAi) pathway. Viruses evade antiviral RNAi by expressing virulence factors known as viral suppressors of RNAi (VSR). Here, we report the identification of VINR, a Drosophila VSR-interacting long non-coding (lnc) RNA that activates non-canonical innate immune signaling upon detection of the dsRNA-binding VSR of Drosophila C virus (DCV). VINR is required for the induction of antimicrobial peptide (AMP) genes but dispensable for antiviral RNAi. VINR functions by preventing the ubiquitin proteasome-dependent degradation of Cactin, a coiled-coil and arginine-serine-rich domain-containing protein that regulates a non-cannonical antimicrobial pathway for AMP induction. CRISPR-Cas9 knockout of VINR in Drosophila cells enhances DCV replication independently of antiviral RNAi, and VINR-knockout adult flies exhibit enhanced disease susceptibility to DCV and bacteria. Our findings reveal a counter counter-defense strategy activated by a lncRNA in response to the viral suppression of the primary antiviral RNAi immunity. |
| Databáze: | OpenAIRE |
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