Lycium barbarum polysaccharides protects retinal ganglion cells against oxidative stress injury
| Autor: | Jing-Xiang Zhong, Yi-Ning Wu, Lian Liu, Meng-Ting Chen, Xiao-Yuan Sha |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
caspase Pharmacology medicine.disease_cause Retinal ganglion lcsh:RC346-429 Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound mitochondrial membrane potential 0302 clinical medicine Developmental Neuroscience medicine lcsh:Neurology. Diseases of the nervous system reactive oxygen species chemistry.chemical_classification Reactive oxygen species cell apoptosis Hypoxia (medical) cobalt chloride Lycium barbarum polysaccharides 030104 developmental biology medicine.anatomical_structure retinal ganglion cells Retinal ganglion cell chemistry Apoptosis oxidative stress injury DNA fragmentation lycium barbarum polysaccharides medicine.symptom 030217 neurology & neurosurgery Oxidative stress Research Article |
| Zdroj: | Neural Regeneration Research, Vol 15, Iss 8, Pp 1526-1531 (2020) Neural Regeneration Research |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.274349 |
| Popis: | The accumulation of excessive reactive oxygen species can exacerbate any injury of retinal tissue because free radicals can trigger lipid peroxidation, protein damage and DNA fragmentation. Increased oxidative stress is associated with the common pathological process of many eye diseases, such as glaucoma, diabetic retinopathy and ischemic optic neuropathy. Many studies have demonstrated that Lycium barbarum polysaccharides (LBP) protects against oxidative injury in numerous cells and tissues. For the model of hypoxia we used cultured retinal ganglion cells and induced hypoxia by incubating with 200 µM cobalt chloride (CoCl2) for 24 hours. To investigate the protective effect of LBP and its mechanism of action against oxidative stress injury, the retinal tissue was pretreated with 0.5 mg/mL LBP for 24 hours. The results of flow cytometric analysis showed LBP could effectively reduce the CoCl2-induced retinal ganglion cell apoptosis, inhibited the generation of reactive oxygen species and the reduction of mitochondrial membrane potential. These findings suggested that LBP could protect retinal ganglion cells from CoCl2-induced apoptosis by reducing mitochondrial membrane potential and reactive oxygen species. |
| Databáze: | OpenAIRE |
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