99mTc-Labeled Superparamagnetic Iron Oxide Nanoparticles for Multimodality SPECT/MRI of Sentinel Lymph Nodes
| Autor: | Sven-Erik Strand, Johan Olsrud, Linda Knutsson, Freddy Ståhlberg, Christian Ingvar, Jimmy Lätt, Fredrik Olsson, Sarah Fredriksson, Renata Madru, Pontus Kjellman, Karin Wingårdh |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Quality Control Pathology medicine.medical_specialty Superparamagnetic iron oxide nanoparticles Sentinel lymph node Contrast Media Spleen Polyethylene glycol Ferric Compounds Polyethylene Glycols Magnetics chemistry.chemical_compound Subcutaneous injection Microscopy Electron Transmission In vivo Image Processing Computer-Assisted medicine Animals Tissue Distribution Radiology Nuclear Medicine and imaging Particle Size Rats Wistar Magnetite Nanoparticles Tomography Emission-Computed Single-Photon Sentinel Lymph Node Biopsy Chemistry business.industry Melanoma Technetium medicine.disease Magnetic Resonance Imaging Rats medicine.anatomical_structure Nanoparticles Chromatography Thin Layer Lymph Nodes Lymph Pharmaceutical Vehicles Radiopharmaceuticals Nuclear medicine business |
| Zdroj: | Journal of Nuclear Medicine. 53:459-463 |
| ISSN: | 2159-662X 0161-5505 |
| Popis: | The purpose of this study was to develop multimodality SPECT/MRI contrast agents for sentinel lymph node (SLN) mapping in vivo. METHODS: Nanoparticles with a solid iron oxide core and a polyethylene glycol coating were labeled with (99m)Tc. The labeling efficiency was determined with instant thin-layer chromatography and magnetic separation. The stability of the radiolabeled superparamagnetic iron oxide nanoparticles (SPIONs) was verified in both sterile water and human serum at room temperature 6 and 24 h after labeling. Five Wistar rats were injected subcutaneously in the right hind paw with (99m)Tc-SPIONs (25-50 MBq, ∼0.2 mg of Fe) and sacrificed 4 h after injection. Two animals were imaged with SPECT/MRI. All 5 rats were dissected; the lymph nodes, liver, kidneys, spleen, and hind paw containing the injection site were removed and weighed; and activity in the samples was measured. The microdistribution within the lymph nodes was studied with digital autoradiography. RESULTS: The efficiency of labeling of the SPIONs was 99% 6 h after labeling in both water and human serum. The labeling yield was 98% in water and 97% in human serum 24 h after labeling. The SLN could be identified in vivo with SPECT/MRI. The accumulation of (99m)Tc-SPIONs (as the percentage injected dose/g [%ID/g]) in the SLN was 100 %ID/g, whereas in the liver and spleen it was less than 2 %ID/g. Digital autoradiography images revealed a nonhomogeneous distribution of (99m)Tc-SPIONs within the lymph nodes; nanoparticles were found in the cortical, subcapsular, and medullary sinuses. CONCLUSION: This study revealed the feasibility of labeling SPIONs with (99m)Tc. The accumulation of (99m)Tc-SPIONs in lymph nodes after subcutaneous injection in animals, verified by SPECT/MRI, is encouraging for applications in breast cancer and malignant melanoma. (Less) |
| Databáze: | OpenAIRE |
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