Detection of Methanobrevobacter smithii and Methanobrevibacter oralis in Lower Respiratory Tract Microbiota
| Autor: | Michel Drancourt, Gérard Aboudharam, Fabienne Brégeon, Yasmine Hassani, Ghiles Grine |
|---|---|
| Přispěvatelé: | Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) animal structures [SDV]Life Sciences [q-bio] 030106 microbiology ved/biology.organism_classification_rank.species Microbiology Methanobrevibacter smithii 03 medical and health sciences Virology medicine microbiota methanogens lcsh:QH301-705.5 medicine.diagnostic_test biology ved/biology Communication biology.organism_classification medicine.disease respiratory tract Methanobrevibacter oralis 3. Good health 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure lcsh:Biology (General) Sputum medicine.symptom Dysbiosis Archaea Respiratory tract Fluorescence in situ hybridization |
| Zdroj: | Microorganisms Microorganisms, 2020, 8 (12), ⟨10.3390/microorganisms8121866⟩ Microorganisms, MDPI, 2020, 8 (12), ⟨10.3390/microorganisms8121866⟩ Microorganisms, Vol 8, Iss 1866, p 1866 (2020) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms8121866⟩ |
| Popis: | Methanogens, the sole microbes producing methane, are archaea commonly found in human anaerobic microbiota. Methanogens are emerging as opportunistic pathogens associated with dysbiosis and are also detected and cultured in anaerobic abscesses. Their presence in the respiratory tract is yet unknown. As a preliminary answer, prospective investigation of 908 respiratory tract samples using polyphasic approach combining PCR-sequencing, real-time PCR, fluorescent in situ hybridization (FISH), and methanogens culture was carried out. Methanobrevibacter smithii and Methanobrevibacter oralis DNA sequences, were detected in 21/527 (3.9%) sputum samples, 2/188 (1.06%) bronchoalveolar lavages, and none of 193 tracheo-bronchial aspirations. Further, fluorescence in situ hybridization detected methanogens in three sputum investigated specimens with stick morphology suggesting M. oralis and in another one bronchoalveolar lavage sample investigated, diplococal morphology suggesting M. smithii. These observations extend the known territory of methanogens to the respiratory tract and lay the foundations for further interpretation of their detection as pathogens in any future cases of isolation from bronchoalveolar lavages and the lungs. |
| Databáze: | OpenAIRE |
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