Rhizobactin B is the preferred siderophore by a novel Pseudomonas isolate to obtain iron from dissolved organic matter in peatlands
| Autor: | Stefan Kügler, Johanna Boessneck, Rebecca E. Cooper, Thomas Wichard, Kirsten Küsel |
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| Rok vydání: | 2020 |
| Předmět: |
Siderophore
Biogeochemical cycle Peat Magnetic Resonance Spectroscopy Peatland Iron Siderophores General Biochemistry Genetics and Molecular Biology Article Biomaterials Metal 03 medical and health sciences Tandem Mass Spectrometry Pseudomonas Dissolved organic carbon Dissolved organic matter 030304 developmental biology Metallophore 0303 health sciences biology Molecular Structure 030306 microbiology Chemistry Metals and Alloys Nuclear magnetic resonance spectroscopy biology.organism_classification visual_art Environmental chemistry visual_art.visual_art_medium General Agricultural and Biological Sciences Bacteria |
| Zdroj: | Biometals |
| ISSN: | 1572-8773 |
| Popis: | Bacteria often release diverse iron-chelating compounds called siderophores to scavenge iron from the environment for many essential biological processes. In peatlands, where the biogeochemical cycle of iron and dissolved organic matter (DOM) are coupled, bacterial iron acquisition can be challenging even at high total iron concentrations. We found that the bacterium Pseudomonas sp. FEN, isolated from an Fe-rich peatland in the Northern Bavarian Fichtelgebirge (Germany), released an unprecedented siderophore for its genus. High-resolution mass spectrometry (HR-MS) using metal isotope-coded profiling (MICP), MS/MS experiments, and nuclear magnetic resonance spectroscopy (NMR) identified the amino polycarboxylic acid rhizobactin and a novel derivative at even higher amounts, which was named rhizobactin B. Interestingly, pyoverdine-like siderophores, typical for this genus, were not detected. With peat water extract (PWE), studies revealed that rhizobactin B could acquire Fe complexed by DOM, potentially through a TonB-dependent transporter, implying a higher Fe binding constant of rhizobactin B than DOM. The further uptake of Fe-rhizobactin B by Pseudomonas sp. FEN suggested its role as a siderophore. Rhizobactin B can complex several other metals, including Al, Cu, Mo, and Zn. The study demonstrates that the utilization of rhizobactin B can increase the Fe availability for Pseudomonas sp. FEN through ligand exchange with Fe-DOM, which has implications for the biogeochemical cycling of Fe in this peatland. Electronic supplementary material The online version of this article (10.1007/s10534-020-00258-w) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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