Effect of Hydroxyapatite Formation on Titanium Surface with Bone Morphogenetic Protein-2 Loading through Electrochemical Deposition on MG-63 Cells

Autor:	Chung Kwei Lin, Huei Yu Huang, Wan Ning Huang, Yankuba B. Manga, Ching Li Tseng, Haw Ming Huang, Chia Yu Wu, Chi Chang Wu
Rok vydání:	2018
Předmět:	Materials science chemistry.chemical_element 02 engineering and technology engineering.material 010402 general chemistry lcsh:Technology 01 natural sciences Bone morphogenetic protein 2 Osseointegration Article calcium phosphate Crystallinity Coating stomatognathic system bone regeneration General Materials Science lcsh:Microscopy Bone regeneration lcsh:QC120-168.85 lcsh:QH201-278.5 lcsh:T hydroxyapatite 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Chemical engineering lcsh:TA1-2040 engineering Alkaline phosphatase lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:TK1-9971 Layer (electronics) Titanium electrochemical deposition
Zdroj:	Materials Volume 11 Issue 10 Materials, Vol 11, Iss 10, p 1897 (2018)
ISSN:	1996-1944
Popis:	Calcium phosphate ceramics used in dentistry and orthopedics are some of the most valuable biomaterials, owing to their excellent osteoconduction, osteoinduction, and osseointegration. Osteoconduction and osteoinduction are critical targets for bone regeneration, and osseointegration is essential for any dental implantations. In this study, a hydroxyapatite (HAp) hybrid coating layer with the sequential release of bone morphogenetic protein 2 (BMP-2) was deposited onto an etched titanium substrate by electrochemical deposition. The resulting release of BMP-2 from Ti&ndash HAp was assessed by immersing samples in a simulated buffer fluid solution. Through coculture, human osteosarcoma cell proliferation and alkaline phosphatase activity were assessed. The characteristics and effect on cell proliferation of the hybrid coatings were investigated for their functionality through X-ray diffraction (XRD) and cell proliferation assays. Findings revealed that &minus 0.8 V vs. Ag/AgCl (3 M KCl) exhibited the optimal HAp properties and a successfully coated HAp layer. XRD confirmed the crystallinity of the deposited HAp on the titanium surface. Ti-0.8 V Ti&ndash HAp co-coating BMP sample exhibited the highest cell proliferation efficiency and was more favorable for cell growth. A successful biocompatible hybrid coating with optimized redox voltage enhanced the osseointegration process. The findings suggest that this technique could have promising clinical applications to enhance the healing times and success rates of dental implantation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec9efaf85e4db7b1613897ce54b0a9ba https://pubmed.ncbi.nlm.nih.gov/30287747 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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