Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan
| Autor: | David Siegel, Vakil Takhaveev, Georges E. Janssens, Georg Hubmann, Anne C. Meinema, Athanasios Litsios, Simeon Leupold, Bastian Niebel, Alexandros Papagiannakis, Matthias Heinemann |
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| Přispěvatelé: | Molecular Systems Biology, Analytical Biochemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Life Sciences & Biomedicine - Other Topics
FLUX QH301-705.5 Systems biology Science Saccharomyces cerevisiae S. cerevisiae General Biochemistry Genetics and Molecular Biology REDOX HOMEOSTASIS Transcriptome 03 medical and health sciences 0302 clinical medicine Metabolome TRANSCRIPTION Biology (General) skin and connective tissue diseases metabolic modelling STRESS-RESPONSE Biology 030304 developmental biology chemistry.chemical_classification HALLMARKS 0303 health sciences Reactive oxygen species Science & Technology General Immunology and Microbiology biology Chemistry General Neuroscience General Medicine Metabolism Cell Biology biology.organism_classification replicative aging Phenotype Cell biology RESPIRATION Proteome Medicine metabolome sense organs Research Advance Life Sciences & Biomedicine 030217 neurology & neurosurgery metabolic fluxes Computational and Systems Biology |
| Zdroj: | eLife, Vol 8 (2019) eLife eLife, 8:e41046. ELIFE SCIENCES PUBLICATIONS LTD eLife, 8 |
| ISSN: | 2050-084X |
| Popis: | A comprehensive description of the phenotypic changes during cellular aging is key towards unraveling its causal forces. Previously, we mapped age-related changes in the proteome and transcriptome (Janssens et al., 2015). Here, employing the same experimental procedure and model-based inference, we generate a comprehensive account of metabolic changes during the replicative life of Saccharomyces cerevisiae. With age, we found decreasing metabolite levels, decreasing growth and substrate uptake rates accompanied by a switch from aerobic fermentation to respiration, with glycerol and acetate production. The identified metabolic fluxes revealed an increase in redox cofactor turnover, likely to combat increased production of reactive oxygen species. The metabolic changes are possibly a result of the age-associated decrease in surface area per cell volume. With metabolism being an important factor of the cellular phenotype, this work complements our recent mapping of the transcriptomic and proteomic changes towards a holistic description of the cellular phenotype during aging. eLife, 8 ISSN:2050-084X |
| Databáze: | OpenAIRE |
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