Automated capillary liquid chromatography for simultaneous determination of neuroactive amines and amino acids
| Autor: | Rebecca D. Rostand, Jacinth A.M McKenzie, Robert T. Kennedy, Steven R. Witowski, Christopher J. Watson, Igor German |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Microdialysis Taurine Sensitivity and Specificity Biochemistry High-performance liquid chromatography Analytical Chemistry Rats Sprague-Dawley Automation chemistry.chemical_compound Biogenic amine Animals Amines Amino Acids Derivatization Brain Chemistry chemistry.chemical_classification Chromatography Organic Chemistry Reproducibility of Results General Medicine Amperometry Rats Amino acid chemistry Butterflies Quantitative analysis (chemistry) Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 962:105-115 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(02)00533-2 |
| Popis: | A method for the separation and quantitative determination of neuroactive amino acids (aspartate, glutamate, citrulline, arginine, glycine, taurine, gamma-aminobutyric acid) and neuroactive amines (noradrenaline, dopamine and serotonin) in a single chromatographic analysis is presented. The method is based on pre-column derivatization with o-phthalaldehyde and tert.-butyl thiol, on-column preconcentration and separation using 50 microns I.D. packed capillary columns, and detection by amperometry. Mass limits of detection are 80-900 amol for all neurotransmitters with RSDs of 0.71 and 4.6% or better for retention time and peak area, respectively. The method was demonstrated by application to the determination of neurotransmitters in microdialysis samples collected from striatum of live rats and tissue samples extracted from butterfly brains. |
| Databáze: | OpenAIRE |
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