Toxoplasma gondiiInfection Potentiates Cognitive Impairments of Alzheimer's Disease in the BALB/c Mice
| Autor: | Vahid Sheibani, Seyed Reza Mirbadie, S Shojaee, Hossein Mahmoudvand, Naser Ziaali, Alireza Keyhani, Hossein Keshavarz, Khadijeh Esmaeelpour |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Spatial Learning Nitric Oxide Synthase Type II Morris water navigation task Hippocampus Real-Time Polymerase Chain Reaction BALB/c Mice Random Allocation 03 medical and health sciences 0302 clinical medicine Immune system Alzheimer Disease Memory parasitic diseases medicine Animals RNA Messenger Maze Learning Swimming Ecology Evolution Behavior and Systematics Neuroinflammation Spatial Memory Mice Inbred BALB C Amyloid beta-Peptides biology Brain Toxoplasma gondii biology.organism_classification medicine.disease Virology Toxoplasmosis Nitric oxide synthase Disease Models Animal Toxoplasmosis Animal 030104 developmental biology Immunology biology.protein Cytokines Parasitology 030217 neurology & neurosurgery |
| Zdroj: | Journal of Parasitology. 102:629-635 |
| ISSN: | 1937-2345 0022-3395 |
| DOI: | 10.1645/16-28 |
| Popis: | This study tests the hypothesis that in chronic Toxoplasma gondii infection communication among immune cells promotes neuroinflammation through cytokine networks and potentiate cognitive impairments in BALB/c mice with Alzheimer's disease (AD). The animal model of Toxoplasma infection was established by the intraperitoneal inoculation of 20–25 tissue cysts from the Tehran strain of T. gondii. We injected amyloid-beta 1–42 peptide (Aβ1–42, 1 and 2 μl) into the hippocampus of BALB/c mice to establish an animal model of AD. The behavioral experiments such as spatial learning and memory were performed using the Morris water maze test. The mRNA levels of TNF-α, IL-1β, IFN-γ, and inducible nitric oxide synthase (iNOS) were examined by real-time PCR. We found that T. gondii infection caused AD-like symptoms and impaired learning and memory functions of the infected BALB/c mice. We also found that in Toxoplasma infection + Aβ1–42 (1 μl) group, T. gondii infection could potentiate AD in infected mice rece... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|