Single-chain variable fragments antibody specific to Corynespora cassiicola toxin, cassiicolin, reduces necrotic lesion formation in Hevea brasiliensis

Autor: Frédéric De Lamotte, Valérie Pujade-Renaud, Rusni A. Kadir, Hoong Yeet Yeang, E. Sunderasan, Sheila Nathan
Přispěvatelé: Rubber Research Institute of Malaya, Universiti Kebangsaan Malaysia, Développement et amélioration des plantes (UMR DAP), Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Plant Science
medicine.disease_cause
Toxine
law.invention
F30 - Génétique et amélioration des plantes
law
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Corynespora cassiicola
0303 health sciences
biology
DETACHED HEVEA LEAF BIOASSAY
Hemagglutinin
respiratory system
Hevea brasiliensis
Recombinant DNA
Réaction antigène anticorps
Protéine recombinante
CASSIICOLIN
chemical and pharmacologic phenomena
Biopanning
Microbiology
03 medical and health sciences
medicine
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
mécanisme de défense
030304 developmental biology
H20 - Maladies des plantes
Génie génétique
Glycoprotéine
030306 microbiology
Toxin
Anticorps
biology.organism_classification
Corynespora
ANTI-CASSIICOLIN SCFV
Protéine microbiologique
Agronomy and Crop Science
Hevea
Zdroj: Journal of General Plant Pathology
Journal of General Plant Pathology, Springer Verlag, 2009, 15 (1), pp.19-26. ⟨10.1007/s10327-008-0137-x⟩
ISSN: 1345-2630
1610-739X
DOI: 10.1007/s10327-008-0137-x⟩
Popis: Correspondance: sunderasan@lgm.gov.my. UMR DAP équipe DGB; International audience; Corynespora leaf disease poses a serious threat to rubber cultivation because infected leaves develop necrotic lesions and abscise, leaving the tree unproductive. The destructiveness of Corynespora cassiicola has been largely attributed to cassiicolin, a protein toxin secreted by the fungus. Recombinant antibody technology offers hope to curtail the disease whereby single-chain variable fragments (scFv) specific to cassiicolin could bind and deactivate the toxin in genetically modified rubber trees that harbour the antibody gene. A scFv phage library was constructed from heavy and light variable chains of IgG from cassiicolin immunized Balb/C mice spleen. Biopanning of the phage library yielded a scFv clone with high specificity to cassiicolin. The nucleotide sequence and deduced amino acid sequence information of the scFv were obtained. Hemagglutinin (HA)-tagged scFv expressed in Escherichia coli is discerned as a band at ca. 30 kDa on SDS-PAGE, and the corresponding band was detected by anti-HA IgG on a Western immunoblot. Deactivation of cassiicolin by the affinity-purified scFv was demonstrated in a detached-leaf bio-assay on selected susceptible Hevea clones (PB 260, RRIM 2020, RRIM 901 and RRIM 929). The assay was also performed on clones that are relatively more resistant to the fungus (RRIM 600 and GT-1), and the results were as expected. Thus, we have successfully demonstrated that the cassicolin-specific scFv can effectively reduce cassicolin toxicity
Databáze: OpenAIRE
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