Multi‐residue extraction to determine organic pollutants in mussel hemolymph
| Autor: | Yolanda Picó, Julián Campo, R. Álvarez-Ruiz |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat Valenciana, European Commission, Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2021 |
| Předmět: |
Illicit drugs
Filtration and Separation 010501 environmental sciences Mass spectrometry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Hemolymph Animals Solid phase extraction Pesticides Acetonitrile 0105 earth and related environmental sciences Detection limit Solid-phase extraction Fluorocarbons Chromatography Illicit Drugs 010401 analytical chemistry Extraction (chemistry) Pesticide 0104 chemical sciences Perfluoroalkyl substances chemistry Pharmaceuticals Environmental Pollutants Methanol |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1615-9314 1615-9306 |
| DOI: | 10.1002/jssc.202001211 |
| Popis: | This study assesses the extraction of eleven pharmaceuticals, five pesticides, five perfluoroalkyl substances, and two illicit drugs in hemolymph from (Mytilus Galloprovincialis). Four extraction procedures using Phree™ Phospholipid Removal cartridges were tested using different volumes of methanol (400 and 600 μL) and acetonitrile (300 and 450 μL). The pollutants were determined by high-performance liquid chromatography–tandem mass spectrometry. The use of methanol gave several problems during the extraction procedure, such as longer times and sample loss. Three methods (acetonitrile 300 and 450 μL; and methanol 600 μL) were validated. Recoveries at three concentration levels (5, 50, and 100 ng/mL) ranged 35.1–129.0 and 29.3–133.0% for acetonitrile 300 and 450 μL, respectively, while recoveries for methanol 600 μL ranged 52.2–166.0%. Limits of detection were < 10 ng/mL for most analytes using any of the methods. Methanol 600 μL was the only method capable to extract the illicit drug 4-methoxyphencyclidine and provided a better peak shape and higher signal-noise ratio. When applied to non-spiked samples from local markets salicylic acid and diclofenac were detected at 33.50-97.79 and 28.30-30.31 ng/mL respectively. To our knowledge, there are no methods to determine organic contaminants in hemolymph and this is the first application of Phree™ cartridges for mussel hemolymph extraction. The research that led to these results received funding from the Spanish Ministry of Science, Innovation and Universities and the European Regional Development Fund through the project WETANDPAC (RTI2018-097158-B-C31) and from the Generalitat Valenciana through the project ANTROPOCEN@ (PROMETEO/2018/155). R. Álvarez-Ruiz acknowledges the Spanish Ministry of Science, Innovation, and Universities and the ERDF (European Regional Development Fund) for his FPI grant BES-2016-078612. |
| Databáze: | OpenAIRE |
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