HIV-1 Antiviral Activity of Recombinant Natural Killer Cell Enhancing Factors, NKEF-A and NKEF-B, Members of the Peroxiredoxin Family
| Autor: | Andrew D. Luster, Hungyi Shau, Marylyn M. Addo, Ralf Geiben-Lynn, Mischo Kursar, Judy Lieberman, Bruce D. Walker, Nancy V. Brown |
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| Rok vydání: | 2003 |
| Předmět: |
Chemokine
Gene Expression CCL1 CD8-Positive T-Lymphocytes Transfection Virus Replication medicine.disease_cause Biochemistry Natural killer cell law.invention Jurkat Cells law Gene expression medicine Humans Molecular Biology Heat-Shock Proteins biology Blood Proteins Peroxiredoxins Cell Biology Simian immunodeficiency virus Virology Recombinant Proteins Killer Cells Natural medicine.anatomical_structure Peroxidases HIV-1 biology.protein Recombinant DNA Peroxiredoxin CD8 |
| Zdroj: | Journal of Biological Chemistry. 278:1569-1574 |
| ISSN: | 0021-9258 |
| Popis: | CD8(+) T-cells are a major source for the production of non-cytolytic factors that inhibit HIV-1 replication. In order to characterize further these factors, we analyzed gene expression profiles of activated CD8(+) T-cells using a human cDNA expression array containing 588 human cDNAs. mRNA for the chemokine I-309 (CCL1), the cytokines granulocyte-macrophage colony-stimulating factor and interleukin-13, and natural killer cell enhancing factors (NKEF) -A and -B were up-regulated in bulk CD8(+) T-cells from HIV-1 seropositive individuals compared with seronegative individuals. Recombinant NKEF-A and NKEF-B inhibited HIV-1 replication when exogenously added to acutely infected T-cells at an ID(50) (dose inhibiting HIV-1 replication by 50%) of approximately 130 nm (3 microg/ml). Additionally, inhibition against dual-tropic simian immunodeficiency virus and dual-tropic simian-human immunodeficiency virus was found. T-cells transfected with NKEF-A or NKEF-B cDNA were able to inhibit 80-98% HIV-1 replication in vitro. Elevated plasma levels of both NKEF-A and NKEF-B proteins were detected in 23% of HIV-infected non-treated individuals but not in persons treated with highly active antiviral therapy or uninfected persons. These results indicate that the peroxiredoxin family members NKEF-A and NKEF-B are up-regulated in activated CD8(+) T-cells in HIV infection, and suggest that these antioxidant proteins contribute to the antiviral activity of CD8(+) T-cells. |
| Databáze: | OpenAIRE |
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