MicroRNA Profiling in Adipose Before and After Weight Loss Highlights the Role of miR‐223‐3p and the NLRP3 Inflammasome
| Autor: | Odette M. Shaw, Alice Johnston, Jacquie L. Harper, Donia Macartney-Coxson, Ronald D. Hagan, Jane Clapham, Richard S. Stubbs, Angela Jones, Kirsty Danielson, Miles C. Benton, Eric P. Hoffman, Michael A. Langston, Mark T. Hayes |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male medicine.medical_specialty Inflammasomes Endocrinology Diabetes and Metabolism Medicine (miscellaneous) Adipose tissue 030209 endocrinology & metabolism medicine.disease_cause Article 03 medical and health sciences 0302 clinical medicine Endocrinology mir-223 Downregulation and upregulation Internal medicine NLR Family Pyrin Domain-Containing 3 Protein Weight Loss microRNA medicine Humans Obesity 030212 general & internal medicine Nutrition and Dietetics biology business.industry Gastric bypass surgery Inflammasome Middle Aged MicroRNAs DNA methylation biology.protein Female business GLUT4 medicine.drug |
| Zdroj: | Obesity (Silver Spring) |
| ISSN: | 1930-739X 1930-7381 |
| DOI: | 10.1002/oby.22722 |
| Popis: | Objective Adipose tissue plays a key role in obesity-related metabolic dysfunction. MicroRNA (miRNA) are gene regulatory molecules involved in intercellular and inter-organ communication. It was hypothesized that miRNA levels in adipose tissue would change after gastric bypass surgery and that this would provide insights into their role in obesity-induced metabolic dysregulation. Methods miRNA profiling (Affymetrix GeneChip miRNA 2.0 Array) of omental and subcutaneous adipose (n = 15 females) before and after gastric bypass surgery was performed. Results One omental and thirteen subcutaneous adipose miRNAs were significantly differentially expressed after gastric bypass, including downregulation of miR-223-3p and its antisense relative miR-223-5p in both adipose tissues. mRNA levels of miR-223-3p targets NLRP3 and GLUT4 were decreased and increased, respectively, following gastric bypass in both adipose tissues. Significantly more NLRP3 protein was observed in omental adipose after gastric bypass (P = 0.02). Significant hypomethlyation of NLRP3 and hypermethylation of miR-223 were observed in both adipose tissues after gastric bypass. In subcutaneous adipose, significant correlations were observed between both miR-223-3p and miR-223-5p and glucose and between NLRP3 mRNA and protein levels and blood lipids. Conclusions This is the first report detailing genome-wide miRNA profiling of omental adipose before and after gastric bypass, and it further highlights the association of miR-223-3p and the NLRP3 inflammasome with obesity. |
| Databáze: | OpenAIRE |
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