Host-Derived Pericytes and Sca-1+ Cells Predominate in the MART-1− Stroma Fraction of Experimentally Induced Melanoma
| Autor: | Taturo Udagawa, Magda E. Bortoni, Douglas A. Cotanche, Rick A. Rogers, J. Humberto Treviño-Villarreal, Otto Manneberg, Rosalinda Sepulveda |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Cell type
Pathology medicine.medical_specialty Histology Stromal cell Time Factors Population CD34 Melanoma Experimental CD146 Antigen Mice SCID Biology Mice MART-1 Antigen Stroma Cell Line Tumor medicine Tumor Microenvironment Animals Antigens Ly education Muscle Skeletal neoplasms Skin education.field_of_study Mice Inbred ICR Epidermis (botany) Neovascularization Pathologic Melanoma Membrane Proteins Mesenchymal Stem Cells Articles medicine.disease Flow Cytometry Hypoxia-Inducible Factor 1 alpha Subunit Immunohistochemistry Cell Hypoxia Cell biology CD146 Endothelium Vascular Anatomy Stromal Cells Pericytes Neoplasm Transplantation |
| Popis: | Identification of cell types in tumor-associated stroma that are involved in the development of melanoma is hampered by their heterogeneity. The authors used flow cytometry and immunohistochemistry to demonstrate that anti–MART-1 antibodies can discriminate between melanoma and stroma cells. They investigated the cellular composition of the MART-1−, non-hematopoietic melanoma-associated stroma, finding it consisted mainly of Sca-1+ and CD146+ cells. These cell types were also observed in the skin and muscle adjacent to developing melanomas. The Sca-1+ cell population was observed distributed in the epidermis, hair follicle bulges, and tumor capsule. The CD146+ population was found distributed within the tumor, mainly associated with blood vessels in a perivascular location. In addition to a perivascular distribution, CD146+ cells expressed α-smooth muscle actin, lacked expression of endothelial markers CD31 and CD34, and were therefore identified as pericytes. Pericytes were found to be associated with CD31+ endothelial cells; however, some pericytes were also observed associated with CD31−, MART-1+ B16 melanoma cells that appeared to form blood vessel structures. Furthermore, the authors observed extensive nuclear expression of HIF-1α in melanoma and stroma cells, suggesting hypoxia is an important factor associated with the melanoma microenvironment and vascularization. The results suggest that pericytes and Sca-1+ stroma cells are important contributors to melanoma development. |
| Databáze: | OpenAIRE |
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