Characterization of three human sec14p-like proteins: alpha-tocopherol transport activity and expression pattern in tissues
| Autor: | Jean-Marc Zingg, Roberta Ricciarelli, Clara De Pascale, Alexandra Cueff, Stefano Menini, Elke Reiter, Michel Arock, Adelina Munteanu, Luis Villacorta, Marcel Paris, Angelo Azzi, Petra Kempná, Rita Cipollone |
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| Rok vydání: | 2008 |
| Předmět: |
alpha-Tocopherol
Vitamin E Mitochondria Golgi Sec14-like proteins Phosphatidylinositol kinase Secretion Sec14-like proteins Mitochondrion Biology Biochemistry symbols.namesake ATP Binding Cassette Transporter Subfamily B Member 3 Cell Line Tumor Organelle Golgi Vitamin E Humans Secretion Tissue Distribution ATP Binding Cassette Transporter Subfamily B Member 2 Protein Precursors Phosphatidylinositol kinase Interleukin 4 Biological Transport General Medicine Golgi apparatus Intracellular lipid transport In vitro Peptide Fragments Recombinant Proteins Cell biology Mitochondria Cell culture symbols ATP-Binding Cassette Transporters Interleukin-4 |
| Zdroj: | Biochimie. 90(11-12) |
| ISSN: | 1638-6183 |
| Popis: | Three closely related human sec14p-like proteins (hTAP1, 2, and 3, or SEC14L2, 3, and 4, respectively) have been described. These proteins may participate in intracellular lipid transport (phospholipids, squalene, tocopherol analogues and derivatives) or influence regulatory lipid-dependent events. Here, we show that the three recombinant hTAP proteins associate with the Golgi apparatus and mitochondria, and enhance the in vitro transport of radioactively labeled alpha-tocopherol to mitochondria in the same order of magnitude as the human alpha-tocopherol transfer protein (alpha-TTP). hTAP1 and hTAP2 are expressed in several cell lines, whereas the expression level of hTAP3 is low. Expression of hTAP1 is induced in human umbilical cord blood-derived mast cells upon differentiation by interleukin 4. In tissues, the three hTAPs are detectable ubiquitously at low level; pronounced and localized expression is found for hTAP2 and hTAP3 in the perinuclear region in cerebellum, lung, liver and adrenal gland. hTAP3 is well expressed in the epithelial duct cells of several glands, in ovary in endothelial cells of small arteries as well as in granulosa and thecal cells, and in testis in Leydig cells. Thus, the three hTAPs may mediate lipid uptake, secretion, presentation, and sub-cellular localization in a tissue-specific manner, possibly using organelle- and enzyme-specific docking sites. |
| Databáze: | OpenAIRE |
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