Immunogenicity of recombinant core particles of hepatitis B virus containing epitopes of human immunodeficiency virus 1 core antigen
| Autor: | E.J. Gren, H. Siakkou, Rainer Ulrich, I. Berzin, Ose Vp, P. Pumpen, Galina Borisova, E. J. Gren, Detlev H. Krüger, R. von Baehr, R. Eckert |
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| Rok vydání: | 1992 |
| Předmět: |
Viral Hepatitis Vaccines
Hepatitis B virus Immunogen viruses Recombinant Fusion Proteins Blotting Western Freund's Adjuvant HIV Core Protein p24 HIV Antibodies medicine.disease_cause Virus Epitope Epitopes Mice Virology medicine Escherichia coli Animals Humans Hepatitis B Vaccines Hepatitis B Antibodies AIDS Vaccines Vaccines Synthetic biology Immunogenicity virus diseases General Medicine Group-specific antigen biology.organism_classification Molecular biology Hepatitis B Core Antigens HBcAg Microscopy Electron Hepadnaviridae HIV-1 Plasmids |
| Zdroj: | Archives of virology. 126(1-4) |
| ISSN: | 0304-8608 |
| Popis: | A Gag protein segment of human immunodeficiency virus 1 (HIV-1) has been fused to a C terminally truncated core antigen of hepatitis B virus (HBcAg) using an E. coli expression system. Fusion of 90 amino acids of HIV-1 Gag protein to HBcAg still allowed the formation of capsids presenting on their surface epitopes of HIV-1 core protein, whereas fusion of 317, 189, or 100 amino acids of Gag prevented self-assembly of chimeric particles. Mice immunized with recombinant particles emulsified with Freund's complete adjuvant (CFA) or aluminium hydroxide developed high anti-HBcAg titers. However, anti-HIVp24 antibodies were detected only in mice inoculated with immunogen emulsified with CFA. |
| Databáze: | OpenAIRE |
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