Interferon-Inducible Protein-10 and the Pathogenesis of Cutaneous T-Cell Lymphomas
| Autor: | Andreas H. Sarris, Hal E. Broxmeyer, Albert B. Deisseroth, Madeleine Duvic, Danai D. Daliani, Fernando Cabanillas, Michael Reiss, Mary Crow, Rose Ulmer, William Pugh |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Keratinocytes Male Cancer Research Chemokine Skin Neoplasms T cell Immunocytochemistry Biology Interferon hemic and lymphatic diseases medicine Humans Aged Aged 80 and over Epidermis (botany) Hematology Middle Aged medicine.disease Lymphoma T-Cell Cutaneous Neoplasm Proteins Lymphoma Chemokine CXCL10 medicine.anatomical_structure Oncology Cancer research biology.protein Female Keratinocyte Chemokines CXC Immunostaining medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1029-2403 1042-8194 |
| DOI: | 10.3109/10428199609045718 |
| Popis: | Human interferon-g inducible protein-10 (IP-10), a small basic protein secreted by interferon (INF)-g stimulated keratinocytes, is chemotactic for normal CD4-positive lymphocytes and inhibits early normal and leukemic hemopoietic progenitor proliferation. Cutaneous T-cell lymphoma (CTCL) is an indolent CD4-positive lymphoma characterized by multiple skin relapses before visceral dissemination. We investigated the role of IP-10 in the biology of CTCL by using immunocytochemistry to define IP-10 expression in normal and CTCL skin biopsies. Using purified recombinant (r) IP-10, we generated a rabbit antiserum that recognized and neutralized rIP-10 but did not cross-react with any keratinocyte proteins or any other chemokine. Immunoperoxidase staining of normal epidermis demonstrated that IP-10 was expressed by basal but not by differentiated keratinocytes. The epidermis overlying CTCL lesions was often hyperplastic, IP-10 immunostaining was enhanced compared to normal skin, and extended to the suprabasal keratinocytes in 25 of 26 patients for a frequency of 96%; and 95% confidence interval (CI) of 80% to 100%. However, IP-10 was detectable in the dermal or epidermal lymphoid infiltrates in only three of these 26 patients (12%; 95% Cl, 2% to 39%). Skin clinically free of CTCL demonstrated normal IP-10 immunostaining. In one patient who had matching biopsies performed before and after treatment, IP-10 was initially overexpressed before treatment but was normally expressed when he achieved remission. These results suggest that IP-10 may play a role in the epidermotropism of CTCL. More work is required to determine whether IP-10 stimulates or inhibits CTCL proliferation. A better understanding of the growth controls operating in CTCL may be used to develop curative therapies for this disorder. |
| Databáze: | OpenAIRE |
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