Dried blood spot self-sampling at home is a feasible technique for hepatitis C RNA detection
| Autor: | Anders Boyd, Janke Schinkel, Marc van der Valk, Tamara Prinsenberg, Freke R Zuure, Sjoerd Rebers, Maria Prins |
|---|---|
| Přispěvatelé: | Graduate School, AII - Infectious diseases, APH - Global Health, Medical Microbiology and Infection Prevention, Infectious diseases, APH - Methodology, APH - Personalized Medicine, APH - Societal Participation & Health, APH - Digital Health |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
RNA viruses
Physiology medicine.medical_treatment RNA Stability Hepacivirus medicine.disease_cause Pathology and Laboratory Medicine Gastroenterology Biochemistry 0302 clinical medicine Immunodeficiency Viruses Blood plasma Medicine and Health Sciences 030212 general & internal medicine Saline Multidisciplinary Hepatitis C virus Viral Load Hepatitis C Dried blood spot Body Fluids Nucleic acids Separation Processes Blood Medical Microbiology Viral Pathogens Viruses Medicine RNA Viral 030211 gastroenterology & hepatology Pathogens Anatomy Viral load Research Article medicine.medical_specialty Science Men WHO Have Sex with Men Research and Analysis Methods Microbiology Blood Plasma Specimen Handling 03 medical and health sciences Internal medicine Virology Retroviruses medicine Genetics Humans Microbial Pathogens Flaviviruses business.industry Lentivirus Organisms RNA Biology and Life Sciences HIV Elution Hepatitis viruses People and Places HIV-1 Population Groupings Gene expression Dried Blood Spot Testing Reagent Kits Diagnostic business Viral Transmission and Infection Self sampling Sexuality Groupings |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 4, p e0231385 (2020) PLoS ONE, 15(4):e0231385. Public Library of Science |
| Popis: | To facilitate HCV diagnosis, we developed an HCV-RNA testing service, which involved home-sampled dried blood spots (DBS). The main objective of this study was to evaluate the feasibility of self-sampling at home. Furthermore, to optimise the processing of DBS samples for RNA detection, we evaluated two elution buffers: phosphate-buffered saline (PBS) and L6-buffer. 27 HCV-RNA and 12 HIV-1 RNA positive patients were included. Laboratory spotted DBS (LabDBS) were made by a technician from blood samples drawn at inclusion. Patients received a DBS home-sampling kit and were requested to return their self-sampled DBS (ssDBS) by mail. We compared the RNA load of PBS and L6-eluted labDBS, and of L6-eluted ssDBS, L6-eluted labDBS and plasma. LabDBS load measurements were repeated after 7-13 and 14-21 days to evaluate RNA stability. All 39 plasma samples provided quantifiable RNA loads. In 1/39 labDBS sample, RNA could not be detected (plasma HCV load: 2.98 log10 IU/ml). L6-eluted samples gave a 0.7 log10 and 0.6 log10 higher viral load for HCV and HIV-1 respectively, compared to PBS-eluted samples. Strong correlations were found between labDBS and ssDBS HCV RNA (r = 0.833; mean difference 0.3 log10 IU/mL) and HIV-1 RNA results (r = 0.857; mean difference 0.1 log10 copies/mL). Correlations between labDBS and plasma values were high for HCV (r = 0.958) and HIV-1 (r = 0.844). RNA loads in DBS remained stable over 21 days. Our study demonstrates that self-sampling dried blood spots at home is a feasible strategy for the detection of HCV and HIV-1 RNA. This could facilitate one-step diagnostics and treatment monitoring in communities with high HCV prevalence. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|