Twenty-six circulating antigens and two novel diagnostic candidate molecules identified in the serum of canines with experimental acute toxoplasmosis
| Autor: | Hua-Jing Zhang, Kehe Huang, Yong-Jun Chen, Yuan-Biao Qiao, Quan Wang, Wei Jiang, Yan Xiao, Xue Junxin, Yingchun Liu |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Proteomics
0301 basic medicine congenital hereditary and neonatal diseases and abnormalities 030106 microbiology Circulating antigens Toxoplasma gondii Antibodies Protozoan Acute infection Antigens Protozoan Enzyme-Linked Immunosorbent Assay Disease 03 medical and health sciences Dogs Antigen mental disorders parasitic diseases medicine Animals Dog Diseases Pathogen biology Research biology.organism_classification medicine.disease Virology Toxoplasmosis Toxoplasmosis Animal 030104 developmental biology Infectious Diseases Parasitology Immunology Diagnostic candidates Toxoplasma |
| Zdroj: | Parasites & Vectors |
| ISSN: | 1756-3305 |
| DOI: | 10.1186/s13071-016-1643-x |
| Popis: | Background The protozoan Toxoplasma gondii is a pathogen that causes severe opportunistic disease in a wide range of hosts. Efficient methods to diagnose acute T. gondii infection are essential for the administration of appropriate treatments and to reduce economic losses. In animals with acute infections, circulating antigens (CAgs) were detected as early as two days post-infection; these CAgs were reliable diagnostic indicators of acute infection. However, only a limited number of CAgs have been identified to date. The objective of this study was to identify a broader spectrum of CAgs and to explore novel diagnostic candidates in serum. Methods A canine model of acute toxoplasmiosis was established. For this purpose, six dogs were infected by intraperitoneal inoculation of tachyzoites. The CAgs spectrum in the serum was identified with the immunoprecipitation-shotgun approach. Two CAgs with low homology to other species, coronin protein (TgCOR) and ELMO protein (TgELMO), were heterologously expressed in Escherichia coli. Polyclonal antibodies against these two proteins were prepared, and the presence of these proteins in the serum was verified by Western blotting. The two CAgs were detected and evaluated by indirect ELISA methods. Results The CAgs levels peaked between two and five days after inoculation, and twenty-six CAgs were identified. Western blotting showed the presence of the two proteins in the serum during acute infection. Based on ELISA tests, the two CAgs were detected during acute infection. Conclusions We identified twenty-six CAgs in the serum of canines with experimental acute toxoplasmosis and discovered two novel diagnostic candidates. We also provide new insights into the diagnosis of acute toxoplasmosis. Electronic supplementary material The online version of this article (doi:10.1186/s13071-016-1643-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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