Characterization of an immunodominantGiardia lamblia protein antigen related to alpha giardin
| Autor: | Richard Sherburne, Wanda M. Wenman, Renate U. Meuser, Ruhangiz T. Kilani, Quoc Nyugen, Khalid El-Shewy |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Glycosylation
Molecular Sequence Data Protozoan Proteins Antibodies Protozoan Fluorescent Antibody Technique Antigens Protozoan Flagellum medicine.disease_cause Immunofluorescence Microbiology Immune system Antigen Antibody Specificity Cell Movement medicine Animals Giardia lamblia Amino Acid Sequence Microscopy Immunoelectron chemistry.chemical_classification General Veterinary biology medicine.diagnostic_test Immunodominant Epitopes Tunicamycin General Medicine biology.organism_classification Molecular biology Amino acid Cytoskeletal Proteins Infectious Diseases chemistry Insect Science biology.protein Protozoa Parasitology Antibody Protein Processing Post-Translational Sequence Analysis |
| Zdroj: | Parasitology Research. 79:587-592 |
| ISSN: | 1432-1955 0044-3255 |
| Popis: | The trophozoites of Giardia lamblia possess several protein antigens, predominant among them a protein of approximately 32,000 Da. In the present study, we used monospecific antibodies that recognize this protein to demonstrate its presence on a variety of G. lamblia isolates from human and animal sources. Immune electron microscopy was used to localize 32-kDa antigen on the trophozoite membrane and disk. Immunofluorescent assays employing monospecific antibodies confirmed the presence of 32-kDa antigen on the membrane and disk and its absence on flagella or nuclei. The N-terminal 17 amino acids of the 32-kDa antigen are identical to alpha-1-giardin, a protein component of microribbons on the ventral disk. These results suggest that the 32-kDa immunodominant trophozoite antigen is alpha-1-giardin. |
| Databáze: | OpenAIRE |
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