Possible role of sildenafil in inhibiting rat vas deferens contractions by influencing the purinergic system
| Autor: | Yüksel Kesim, S. Sırrı Bilge, Elif Aksoz, Suleyman Celik, Mehmet Kurt |
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| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty ejaculation Sildenafil Phosphodiesterase Inhibitors Urology Suramin sildenafil In Vitro Techniques purinergic system Piperazines Sildenafil Citrate chemistry.chemical_compound Vas Deferens Internal medicine medicine Animals rat Sulfones Rats Wistar Cyclic guanosine monophosphate Evans Blue business.industry Purinergic receptor Vas deferens Receptors Purinergic Phosphodiesterase Muscle Smooth PDE5 drug design Rats respiratory tract diseases medicine.anatomical_structure Endocrinology chemistry Purines cardiovascular system business medicine.drug Muscle Contraction vas deferens |
| Popis: | Bilge, S.Sirri/0000-0003-2878-6968; WOS: 000232101700008 PubMed: 16201980 Aim: To evaluate the effect of sildenafil, a selective inhibitor of cyclic guanosine monophosphate (cGMP)-selective type 5 phosphodiesterase, on isolated rat vas deferens and its connections with the purinergic system. Methods: Epididymal and prostatic portions of isolated vas deferens were placed in organ baths containing Krebs' solution. Contractions were induced by noradrenaline (NA), adenosine triphosphate (ATP), alpha,beta-methylene ATP and electrical field stimulation (EFS). The effect of sildenafil on the contractions was compared with suramin and Evans blue (EB). Results: NA, ATP, alpha,beta-methylene ATP and EFS caused contractions in both portions of vas deferens. NA-induced contractions were unaffected by sildenafil and suramin but potentiated by EB. ATP-induced contractions were non-competitively inhibited in both portions by sildenafil and suramin but potentiated by EB. alpha,beta-methylene ATP-induced contractions were unaffected by sildenafil but were inhibited in both portions by suramin and EB. EFS-induced contractions were inhibited by sildenafil and suramin while potentiated by EB. Conclusion: Sildenafil inhibited the contractions in both portions of vas deferens, as did suramin. We have suggested that purinergic system has a role in this antagonism and it seems to be mediated by an ATP-dependent mechanism instead of a receptor interaction. |
| Databáze: | OpenAIRE |
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