Diguanosine nucleotides of fungi that regulate RNA polymerases isolated and partially characterised
| Autor: | Herb B. LéJohn, Glen R. Klassen, Linda E. Cameron, David R. McNaughton |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
food.ingredient
Biophysics Guanosine Biochemistry chemistry.chemical_compound food Species Specificity Blastocladiella Protein biosynthesis Nucleotide Pyrophosphatases Molecular Biology chemistry.chemical_classification biology Phosphoric Diester Hydrolases fungi Aspergillus niger Fungi RNA DNA-Directed RNA Polymerases Cell Biology Achlya Hydrogen-Ion Concentration Alkaline Phosphatase biology.organism_classification Guanine Nucleotides chemistry Acid hydrolysis Rhizopus |
| Zdroj: | Biochemical and Biophysical Research Communications. 66:460-467 |
| ISSN: | 0006-291X |
| Popis: | Three unusual phosphorylated diguanosine compounds called ‘hot spots’ HS-1, HS-2 and HS-3 (ref. LeJohn, H.B. Proc. Can. Fed. Biol. Soc. 18, 159, 1975) have been isolated as acid-soluble materials from several fungi, Achlya, Blastocladiella emersonii, Aspergillus niger and Rhizopus stolonifer in their vegetative phase. The nucleotides were purified from acid extracts of Achlya and Blastocladiella. The tentative structures of HS-3 and HS-2 determined are GppppG and GppppGp. HS-1 structure is still in doubt but it is related to HS-2. The structures were deduced from enzymatic digestion and UV analyses of the products, molar ratios of guanosine and phosphate, and chromatographic behaviour on PEI-cellulose. All three compounds accumulated in an inverse manner with rates of RNA synthesis and directly with rates of protein synthesis. The acid-soluble pools of the three compounds fluctuated during the life cycle of Achlya, and just prior to sporulation, were excreted into the medium. HS-2 was convertible to HS-3 by acid hydrolysis. |
| Databáze: | OpenAIRE |
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