Arabidopsis ALF4encodes a nuclear‐localized protein required for lateral root formation
| Autor: | Paula Grisafi, Jill Sheets, Gerald R. Fink, Shane Buker, Raymond J. DiDonato, John L. Celenza, Erin Arbuckle, Ronald Totong, José Tobar |
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| Rok vydání: | 2004 |
| Předmět: |
Molecular Sequence Data
Mutant Arabidopsis Mitosis Plant Science medicine.disease_cause Plant Roots Gene expression Genetics medicine Amino Acid Sequence Cloning Molecular Lateral root formation Gene DNA Primers Cell Nucleus Mutation Base Sequence biology Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Cell Biology Meristem biology.organism_classification Alternative Splicing Pericycle |
| Zdroj: | The Plant Journal. 37:340-353 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1046/j.1365-313x.2003.01964.x |
| Popis: | Lateral root formation, the primary way plants increase their root mass, displays developmental plasticity in response to environmental changes. The aberrant lateral root formation (alf)4-1 mutation blocks the initiation of lateral roots, thus greatly altering root system architecture. We have positionally cloned the ALF4 gene and have further characterized its phenotype. The encoded ALF4 protein is conserved among plants and has no similarities to proteins from other kingdoms. The gene is present in a single copy in Arabidopsis. Using translational reporters for ALF4 gene expression, we have determined that the ALF4 protein is nuclear localized and that the gene is expressed in most plant tissues; however, ALF4 expression and ALF4's subcellular location are not regulated by auxin. These findings taken together with further genetic and phenotypic characterization of the alf4-1 mutant suggest that ALF4 functions independent from auxin signaling and instead functions in maintaining the pericycle in the mitotically competent state needed for lateral root formation. Our results provide genetic evidence that the pericycle shares properties with meristems and that this tissue plays a central role in creating the developmental plasticity needed for root system development. |
| Databáze: | OpenAIRE |
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