Changes in the ratio of branched-chain to aromatic amino acids affect the secretion of albumin in cultured rat hepatocytes
| Autor: | Masataka Okuno, Soichi Kojima, Yoshinori Muto, Michimasa Kato, Hisataka Moriwaki |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Phenylalanine Biophysics Serum albumin Gene Expression Biochemistry chemistry.chemical_compound Biosynthesis Leucine Aromatic amino acids Animals RNA Messenger Isoleucine Rats Wistar Molecular Biology Cells Cultured Serum Albumin chemistry.chemical_classification biology Chemistry Albumin Valine Cell Biology Amino acid Rats Kinetics Liver biology.protein Tyrosine Amino Acids Branched-Chain |
| Zdroj: | Biochemical and biophysical research communications. 214(3) |
| ISSN: | 0006-291X |
| Popis: | The effect of branched-chain amino acids (BCAAs) on the synthesis and secretion of albumin was studied in the primary cultures of rat hepatocytes. The changes in the normal BCAAs/aromatic amino acids (AAAs) ratio reduced the secretion of albumin without altering its mRNA levels. Protein-labeling and pulse-chase experiments showed that a low BCAAs/AAAs ratio reduced the biosynthesis, whereas a high ratio accelerated intracellular decay of albumin. These results suggest that normalization of the BCAAs/AAAs ratio improves albumin synthesis while the excess ratio may induce the degradation of albumin in the cells. |
| Databáze: | OpenAIRE |
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