In vitro modulation of human gingival epithelial cell attachment and migration by minocycline-HCI
| Autor: | S. Zahedi, Charles M. Lapière, Alain Vanheusden, Eric Rompen, Gerhard Goffinet, Betty Nusgens |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Keratinocytes Pathology medicine.medical_specialty Time Factors Adolescent Epithelial Attachment Gingiva Junctional epithelium Connective tissue Minocycline Tritium Cell Movement In vivo Cell Adhesion medicine Humans Cells Cultured Antibacterial agent Wound Healing Chemotactic Factors business.industry Epithelial Cells Cell migration Fetal Blood Molecular biology Epithelium Anti-Bacterial Agents Culture Media Kinetics medicine.anatomical_structure Microscopy Electron Scanning Periodontics Radiopharmaceuticals Wound healing business medicine.drug |
| Zdroj: | Journal of Periodontal Research. 33:377-385 |
| ISSN: | 1600-0765 0022-3484 |
| Popis: | Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated amino-acids. Increasing concentrations of minocycline (10, 50, 100 micrograms/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100 micrograms/ml which statistically significantly (p < 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 micrograms/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 micrograms/ml of minocycline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10 micrograms/ml minocycline-HCl solution increased significantly (p < 0.005) cell migration towards a gradient of fetal calf serum. The presence of 10 micrograms/ml of minocycline in contact with the keratinocytes in the upper compartment of the migration chambers also produced a significant (p < 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemoattractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyond 50 micrograms/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of a junctional epithelium. |
| Databáze: | OpenAIRE |
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