In situ hybridization analysis of synovial and systemic cytokine messenger RNA expression in superantigen-mediated Staphylococcus aureus arthritis
| Autor: | Tomas Olsson, Åke Ljungdahl, Yi-Xue Zhao, Andrej Tarkowski |
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| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_treatment Immunology Bacterial Toxins Molecular Sequence Data Arthritis In situ hybridization Biology Proinflammatory cytokine Enterotoxins Mice Rheumatology Gene expression medicine Superantigen Immunology and Allergy Animals Pharmacology (medical) RNA Messenger In Situ Hybridization Messenger RNA Arthritis Infectious Superantigens Tumor Necrosis Factor-alpha Interleukins Synovial Membrane Staphylococcal Infections medicine.disease Disease Models Animal medicine.anatomical_structure Cytokine Cytokines Female Synovial membrane Spleen |
| Zdroj: | Arthritis and rheumatism. 39(6) |
| ISSN: | 0004-3591 |
| Popis: | OBJECTIVE To investigate patterns of synovial and systemic cytokine messenger RNA (mRNA) expression in mice with superantigen-mediated Staphylococcus aureus arthritis. METHODS Mice were inoculated intravenously with 1 x 10(7) colony-forming units of toxic shock syndrome toxin-1-producing S aureus LS-1. Synovial tissues and spleens were obtained at varying time intervals after bacterial inoculation, and examined for mRNA expression of interleukin-1beta (IL-1beta), IL-4, IL-10, IL-12, tumor necrosis factor alpha (TNFalpha), TNFbeta, interferon-gamma (IFN-gamma), transforming growth factor beta, and perforin, by an in situ hybridization technique. RESULTS In situ hybridization revealed early synovial up-regulation of TNFalpha and IL-1 beta mRNA expression. Peak frequencies of these proinflammatory cytokines were observed at the second and third week of the infection. Expression of T cell-derived cytokine mRNAs was detected later, and in a relatively low frequency. Notably, induction and peak numbers of Th2 cytokine (IL-4 and IL-10) mRNA expression preceded Th1 cytokine (IFNgamma and TNFbeta) mRNAs. In comparison with synovial tissues, peak spleen cytokine mRNA expression of IL-1beta, TNFalpha, TNFbeta, IL-12, and IFNgamma occurred earlier, but displayed a clearly lower magnitude of expression. CONCLUSION These findings demonstrate synovial and systemic up-regulation of cytokine mRNA expression during S aureus arthritis, indicating that both monocyte/macrophage and T cell-derived products are involved in the pathogenesis of this disease. |
| Databáze: | OpenAIRE |
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