Characterization of the expression pattern of the PRC2 core subunitSuz12during embryonic development ofXenopus laevis
| Autor: | Issam Aldiri, Monica L. Vetter |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Nervous system
Embryo Nonmammalian Protein subunit Molecular Sequence Data Xenopus Embryonic Development Polycomb-Group Proteins macromolecular substances Xenopus Proteins Nervous System Article Xenopus laevis Histone H3 Polycomb-group proteins medicine Animals Enhancer of Zeste Homolog 2 Protein Amino Acid Sequence Cloning Molecular Phylogeny Genetics Sequence Homology Amino Acid biology Gene Expression Profiling EZH2 Polycomb Repressive Complex 2 Gene Expression Regulation Developmental Nuclear Proteins biology.organism_classification Repressor Proteins Protein Subunits medicine.anatomical_structure biology.protein Retinoblastoma-Binding Protein 4 PRC2 Neural development Developmental Biology |
| Zdroj: | Developmental Dynamics. 238:3185-3192 |
| ISSN: | 1097-0177 1058-8388 |
| DOI: | 10.1002/dvdy.22120 |
| Popis: | The Polycomb repressive complex 2 is a multimeric aggregate that mediates silencing of a broad range of genes, and is associated with important biological contexts such as stem cell maintenance and cancer progression. PRC2 mainly trimethylates lysine 27 of histone H3 and is composed of three essential core subunits: EZH2, EED, and SUZ12. The Xenopus orthologs of PRC2 subunits Ezh2 and Eed have been described but Suz12 remained unidentified. Here, we report the cloning of the Xenopus Suz12, and determine its spatiotemporal expression during development. Xsuz12 transcript is provided maternally and continues to be expressed throughout development, particularly in the anterior part of the developing central nervous system. Importantly, comparative analysis of the expression of the PRC2 subunits Xez, Xeed, and Xrbbp4 indicates that their expression largely coincides with Xsuz12 in the nervous system, suggesting that PRC2 may have unexplored functions in the development of the frog central nervous system. |
| Databáze: | OpenAIRE |
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