Development of effective antimicrobial cocktails to prevent bacterial contamination of allograft tissues under low temperature conditions
| Autor: | Masanori Sasatsu, Norihisa Noguchi, Hidemasa Nakaminami, Koji Koishikawa, Mana Shioura, Misa Tajima, Yuma Shiratori |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Pulmonary and Respiratory Medicine Microbial Sensitivity Tests 030204 cardiovascular system & hematology medicine.disease_cause Enterococcus faecalis Microbiology 03 medical and health sciences 0302 clinical medicine Levofloxacin Disease Transmission Infectious Humans Surgical Wound Infection Medicine biology business.industry Temperature Staphylococcal Infections biochemical phenomena metabolism and nutrition Allografts bacterial infections and mycoses Antimicrobial biology.organism_classification Anti-Bacterial Agents Drug Combinations 030228 respiratory system Staphylococcus aureus Heart Transplantation Vancomycin Surgery Gentamicin Daptomycin Cardiology and Cardiovascular Medicine business Polymyxin B medicine.drug |
| Zdroj: | Interactive CardioVascular and Thoracic Surgery. 28:128-136 |
| ISSN: | 1569-9285 |
| DOI: | 10.1093/icvts/ivy209 |
| Popis: | Objectives Prevention of bacterial transmission in recipient patients via allograft decontamination with an antimicrobial cocktail consisting of cefmetazole (cefoxitin), vancomycin, lincomycin and polymyxin B is an important procedure commonly practised in tissue banks. However, some allografts are lost due to the failure of decontamination under low temperature conditions. Here, we aimed to develop new antimicrobial cocktails that exert a high bactericidal activity at 4°C. Methods Bacterial species used in this study were selected as major causative pathogens of allograft tissue contamination. The efficacy of the combination of 2 antimicrobial agents was determined by the checkerboard titration method. The bactericidal effects of the new antimicrobial cocktails were evaluated under the same conditions as those used for the storage and preservation of allograft tissues. Results Among the selected antimicrobial agents, daptomycin exhibited the highest bactericidal activity against methicillin-resistant Staphylococcus aureus under low temperature conditions. The combination of daptomycin + gentamicin and daptomycin + levofloxacin showed a synergistic or additive effect against various bacterial species. The antimicrobial cocktail containing 200 μg/ml of daptomycin, gentamicin and levofloxacin could eradicate ≤104 colony-forming units/ml of methicillin-resistant S. aureus and Enterococcus faecalis, which exhibit a low susceptibility to antimicrobial agents at 4°C for 24 h. Conclusions We have developed a new formula for an antimicrobial cocktail to effectively and sufficiently prevent bacterial contamination of allograft tissues under low temperature conditions in vitro. |
| Databáze: | OpenAIRE |
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